真菌性中耳炎患者血清炎症因子及中耳肉芽组织标本TLR2、TLR4蛋白表达与mRNA水平  

Serum inflammatory factors and expression levels of TLR2, TLR4 proteins and mRNA in middle ear granulation tissue specimens of patients with fungal otitis media

作　　者：汪琳 张丹[1] 张庆玲 刘春艳[1] 黄蔓鑫 WANG Lin;ZHANG Dan;ZHANG Qing-ling;LIU Chun-yan;HUANG Man-xin(Tongji Hospital Affiliated to Tongji Medical College of Huazhong University of Science and Technology,Wuhan,Hubei 430030,China;不详)

机构地区：[1]华中科技大学同济医学院附属同济医院耳鼻咽喉头颈外科,湖北武汉430030 [2]江汉大学医学院,湖北武汉430030

出　　处：《中华医院感染学杂志》2022年第2期247-251,共5页Chinese Journal of Nosocomiology

基　　金：湖北省科研计划研究基金资助项目(190211H310)。

摘　　要：目的 探讨真菌性中耳炎患者血清炎症因子及中耳分泌物Toll样受体2(TLR2)、TLR4蛋白表达与信使RNA(mRNA)水平。方法 选取2018年12月-2019年12月收治的真菌性中耳炎患者102例(病例组),无外耳道感染的耳硬化症患者40例(对照组)。检测两组患者血清白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)、巨噬细胞炎症蛋白-2(MIP-2)。术中采集病例组中耳肉芽组织标本及对照组中耳黏膜标本。免疫组化染色检测TLR2与TLR4的蛋白表达;逆转录聚合酶链式反应(RT-PCR)检测TLR2与TLR4 mRNA水平。结果 共检出真菌102株,以曲霉菌35株占34.32%、青霉菌28株占27.45%、白假丝酵母23株占22.55%为主。病例组血清IL-6、TNF-α、MIP-2水平均高于对照组(P<0.05)。病例组中耳肉芽组织TLR2与TLR4的蛋白表达及mRNA水平均高于对照组中耳黏膜标本(P<0.05)。结论 真菌性中耳炎的分子机制可能与TLR2和TLR4基因的表达上调有关。OBJECTIVE To explore the expression levels of serum inflammatory factors and proteins and messenger-ribonucleic acid(mRNA) of Toll-like receptor 2(TLR2) and TLR4 in middle ear secretions of patients with fungal otitis media. METHODS A total of 102 patients with fungal otitis media who were treated from Dec 2018 to Dec 2019 were assigned as the case group, and 40 otosclerosis patients without external ear canal infection were chosen as the control group. The levels of serum interleukin-6(IL-6), tumor necrosis factor-α(TNF-α) and macrophage inflammatory protein-2(MIP-2) were detected, the middle ear granulation tissue specimens were collected from the patients of the case group during surgery, and the middle ear mucosa specimens were collected from the patients of the control group. The expression levels of TLR2 and TLR4 were detected by immunohistochemical staining, and the mRNA levels of TLR2 and TLR4 were detected by means of reverse transcription polymerase chain reaction(RT-PCR). RESULTS Totally 102 strains of fungi were isolated, 35(34.32%) of which were Aspergillus, 28(27.45%) were Penicillium, and 23(22.55%) were Candida albicans. The levels of serum IL-6, TNF-α and MIP-2 of the case group were significantly higher than those of the control group(P<0.05).The expression levels of proteins and mRNA of TLR2 and TLR4 were significantly higher in the middle ear granulation tissue specimens of the case group than in the middle ear mucosa specimens of the control group(P<0.05). CONCLUSION The molecular mechanism of fungal otitis media may be related to the up-regulated expression of TLR2 and TLR4 genes.

关 键 词：真菌性中耳炎 Toll样受体 炎症因子 蛋白表达 信使RNA 

分 类 号：R764.21[医药卫生—耳鼻咽喉科]