miR-150对角叉菜胶诱导慢性非细菌性前列腺炎模型炎症调控作用及机制  

Effect of miR-150 on regulation of inflammation in rat models with Carrageenan-induced chronic nonbacterial prostatitis

作　　者：罗玉叶 唐猛 吴晓飞 蒋萍 段秋霞 沈国球 LUO Yu-ye;TANG Meng;WU Xiao-fei;JIANG Ping;DUAN Qiu-xia;SHEN Guo-qiu(Taikang Tongji(Wuhan)Hospital,Wuhan,Hubei 430050,China)

机构地区：[1]泰康同济(武汉)医院泌尿外科,湖北武汉430050

出　　处：《中华医院感染学杂志》2022年第5期735-739,共5页Chinese Journal of Nosocomiology

基　　金：湖北省卫生健康委员会重点基金资助项目(WJ2019Z007)。

摘　　要：目的 分析微小RNA-150(miR-150)对角叉菜胶诱导慢性非细菌性前列腺炎(CNP)模型的炎症调控作用及相关机制。方法 选取60只SFP级雄性大鼠,随机分为A、B、C、D组各15只,A组不做任何处理,剩余三组大鼠建立角叉菜胶诱导的CNP模型。C组做miR-150沉默转染、D组做miR-150过表达转染,A组、B组注射生理盐水。对比分析各组指标。结果 与A、B组相比,C组miR-150表达量降低,D组miR-150表达量升高(P<0.05)。相比B组,C组体质量、前列腺湿质量较低,D组体质量、前列腺湿质量较高(P<0.05);相比C组,D组体质量、前列腺湿质量较高(P<0.05)。相比B组,C组白细胞介素(IL-1β、IL-6、IL-8)、肿瘤坏死因子-α(TNF-α)水平及p38丝裂原活化蛋白激酶(p38MAPK)、磷酸化p38丝裂原活性蛋白激酶(p-p38MAPK)、基质金属蛋白酶-9(MMP-9)蛋白表达量较高,D组IL-1β、IL-6、IL-8、TNF-α水平及p38MAPK、p-p38MAPK、MMP-9蛋白表达量较低(P<0.05);相比C组,D组IL-1β、IL-6、IL-8、TNF-α水平较低(P<0.05)。结论 miR-150过表达可能经作用于p38MAPK信号通路,最终发挥调控角叉菜胶诱导CNP局部炎症发挥作用。OBJECTIVE To observe the effect of MicroRNA-150(miR-150) on regulation of inflammation in rat models with Carrageenan-induced chronic nonbacterial prostatitis(CNP). METHODS Totally 60 male SFP rats were enrolled and randomly divided into the group A, B, C and D, with 15 rats in each group. The group A was not given any treatment, the rats of the rest three groups were induced by Carrageenan to establish CNP models. The group C was treated with miR-150 silent transfection, the group D was treated with miR-150 overexpression transfection, the group A and the group B were treated with injection of normal saline. The indexes were observed and compared among the groups. RESULTS As compared wit the group A and the group B, the expression level of miR-150 of the group C was reduced significantly, and the expression level of miR-150 of the group D was elevated significantly(P<0.05). As compared with the group B, the body weight and wet weight of prostate of the group C were relatively low, while the body weight and wet weight of prostate of the group D were relatively high(P<0.05). As compared with the group B, the levels of interleukins(IL-1β, IL-6, IL-8), tumor necrosis factor-α(TNF-α) and the expression levels of p38 mitogen-activated protein kinase(p38 MAPK), phosphorylated p38 mitogen-activated protein kinase(p-p38 MAPK)), matrix metalloproteinase-9(MMP-9) proteins were relatively high in the group C(P<0.05), while the levels of IL-1β, IL-6, IL-8, TNF-α and the expression levels of p38 MAPK, p-p38 MAPK and MMP-9 proteins were relatively low in the group C(P<0.05). The levels of IL-1β, IL-6, IL-8 and TNF-α of the group D were significantly lower than those of the group C(P<0.05). CONCLUSION The miR-150 overexpression may eventually regulate the local inflammation of Carrageenan-induced CNP by acting on the p38 MAPK signaling pathway.

关 键 词：微小RNA-150 角叉菜胶 慢性非细菌性前列腺炎 炎症 磷酸化p38丝裂原活性蛋白激酶信号通路 白细胞介素 肿瘤坏死因子-α 

分 类 号：R697.33[医药卫生—泌尿科学]