基于转录组测序红秋葵果实转色期花青素生物合成相关基因研究  

Study on Genes Related to Anthocyanin Biosynthesis at the Color Turning Stages in Red Okra Fruit Based on Transcriptome Sequencing

作　　者：王俊青[1,2] 张彦辉 易婉莹 龚仲观 许贺宾 李艳艳 WANG Junqing;ZHANG Yanhui;YI Wanying;GONG Zhongguan;XU Hebin;LI Yanyan(Pingdingshan Medicinal Plant Functional Component Utilization Engineering Technology Research Center,Pingdingshan University,Pingdingshan,Henan 467000;Henan Provincial Key Laboratory of Ecological and Economical Woody Plant Germplasm Innovation and Utilization,Pingdingshan,Henan 467000)

机构地区：[1]平顶山学院平顶山市药用植物功能成分利用工程技术研究中心,河南平顶山467000 [2]河南省生态经济型木本植物种质创新与利用重点实验室,河南平顶山467000

出　　处：《北方园艺》2022年第14期9-16,共8页Northern Horticulture

基　　金：国家自然科学基金资助项目(31700597);河南省高等学校重点科研资助项目(22B350005)。

摘　　要：以红秋葵果实发育转色期为研究对象,运用Illumina HiSeq 2500高通量测序技术对其转色期进行转录组测序,研究红秋葵果实转色期花青素合成相关基因,以期从差异表达的基因中鉴定得到花青素生物合成的相关酶基因。结果表明:在红秋葵果实发育转色期转录本中共获得208145条Unigenes,对不同果实发育转色期差异表达的基因进行KEGG富集分析,共鉴定到28条花青素生物合成相关的Unigenes。根据其在红秋葵果实发育转色期表达情况,推测CHS4、CHS6、CHS8、HCT1、HCT2、CHI、UFGT和F3′5′H这8条基因在花青素生物合成中起关键作用。The red okra fruit development and color turning periods(three key development periods)were used as research object.The transcriptome of the color turning stages in red okra fruit were sequenced by Illumina HiSeq 2500sequencing technology,the related enzyme genes of anthocyanin biosynthesis were identified from differentially expressed genes.In the transcripts of three key development periods,a total of 208 145 Unigenes were assembled,Kyoto Encyclopedia of Genes and Genomes (KEGG)enrichment analysis of genes differentially expressed in different tissues showed that a total of 28 differentially expressed genes related to anthocyanin synthesis were identified.According to the expression level analysis of genes at the color turning stages in red okra fruit,8Unigenes of CHS4,CHS6,CHS8,HCT1,HCT2,CHI,UFGTand F3′5′H were assummed as the key genes of anthocyanin synthesis.

关 键 词：红秋葵 转录组 花青素生物合成 KEGG代谢途径 

分 类 号：S649[农业科学—蔬菜学]