转cry1A.301基因玉米对主要鳞翅目害虫的抗性研究  

Studies on Resistance of Transgenic cry1A.301 Maize(Zea mays)to Major Lepidopteran Pests

作　　者：杨小艳[1] 王云鹤 翁建峰[3] 吴红[1] 韩垚 雷开荣[1] 谢树章[1] YANG Xiao-Yan;WANG Yun-He;WENG Jian-Feng;WU Hong;HAN Yao;LEI Kai-Rong;XIE Shu-Zhang(Chongqing Key Laboratory of Adversity Agriculture Research,Institute of Biotechnology,Chongqing Academy of Agricultural Sciences,Chongqing 401329,China;College of Agriculture,Northeast Agricultural University,Harbin 150030,China;Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081,China)

机构地区：[1]重庆市农业科学院生物技术研究所/逆境农业研究重庆市市级重点实验室,重庆401329 [2]东北农业大学农学院,哈尔滨150030 [3]中国农业科学院作物科学研究所,北京100081

出　　处：《农业生物技术学报》2022年第7期1247-1256,共10页Journal of Agricultural Biotechnology

基　　金：重庆市农业科学院农业发展资金项目(NKY-2021AB001)。

摘　　要：亚洲玉米螟(Ostrinia furnacalis)、黏虫(Mythimna separata)和棉铃虫(Helicoverpa armigera)是为害玉米(Zea mays)的主要鳞翅目害虫。cry1A.301基因是经优化的抗虫基因。为探讨转cry1A.301基因玉米对亚洲玉米螟、黏虫和棉铃虫的抗性,本研究采用室内玉米离体组织生测和田间人工接虫鉴定相结合的方法,评价了CM8201和CM8204两个转化体对亚洲玉米螟、黏虫和棉铃虫的杀虫效果。室内生测结果表明:CM8201和CM8204的心叶、花丝及籽粒对亚洲玉米螟、棉铃虫、黏虫有很强的毒杀作用,接虫5 d后幼虫死亡率均达95%以上。田间接虫鉴定结果表明:CM8201和CM8204心叶期接虫后,亚洲玉米螟的食叶级别平均值分别为1.17和1.18,虫害级别均为1级,抗性水平均为高抗,黏虫的食叶级别平均值分别为1.38和1.35,抗性水平均为高抗。CM8201和CM8204吐丝期接虫后,亚洲玉米螟雌穗被害级别平均值分别为1.27和1.30,棉铃虫雌穗被害平均值分别为0.15和0.20,抗性水平均为高抗。综上所述,转基因抗虫玉米CM8201和CM8204在各个发育阶段对3种主要玉米鳞翅目害虫均表现出良好的抗虫效果,为抗虫转基因玉米新品种的培育提供了材料基础。Ostrinia furnacalis,Mythimna separata and Helicoverpa armigera are the main lepidopteran pests to corn.In order to assess the resistance of transgenic cry1A.301 maize to these insect pests,two events of CM8201 and CM8204 were evaluated through in vitro tissues laboratory bioassay and artificial infestation in field trials.The results of laboratory bioassay showed that the larval mortality rate of O.furnacalis,M.separate and H.armigera was above 95%when the larvae fed on the heart leaves,silks and grains of CM8201 and CM8204 after 5 d.Hence,the heart leaves,silks and grains of CM8201 and CM8204 showed high resistance to these pests.The results of insect identification on field showed that at whorl stage,as far as the resistance to O.furnacalis be concerned,the mean values of feeding grade of CM8201 and CM8204 were 1.17 and 1.18,respectively.The pest grade was 1.The resistance level was high.As far as the resistance to M.separate be concerned,the mean values of feeding grade of CM8201 and CM8204 were 1.38 and 1.35,respectively,and the resistance type was high.At silking stage,as far as the resistance to O.furnacalis be concerned,the average damage level of female ear of CM8201 and CM8204 was 1.27 and 1.30,respectively.As far as the resistance to H.armigera be concerned,the average damage level of female ear was 0.15 and 0.20,respectively.In conclusion,the transgenic insect-resistant maize CM8201 and CM8204 showed good insecticidal effects against 3 major maize lepidopteran pests at all stages of maize growth.This study provides material basis for the breeding of new insect-resistant transgenic maize varieties.

关 键 词：cry1A.301基因 抗虫玉米 鳞翅目害虫 抗虫性 

分 类 号：S513[农业科学—作物学]