盐胁迫下海马齿生理指标变化及相关基因表达分析  

Physiological Changes and Related Gene Expression Analysis of Sesuvium portulacastrum Under Salt Stress

作　　者：李雨欣 罗秀丽 张婷婷 康宇乾 王鹏[1] 江行玉[2] 周扬[1] LI Yu-Xin;LUO Xiu-Li;ZHANG Ting-Ting;KANG Yu-Qian;WANG Peng;JIANG Xing-Yu;ZHOU Yang(School of Horticulture/Key Laboratory for Quality Regulation of Tropical Horticultural Crops of Hainan Province,Hainan University,Haikou 570228,China;School of Tropical Crops/Hainan Key Laboratory for Biotechnology of Salt Tolerant Crops,Hainan University,Haikou 570228,China)

机构地区：[1]海南大学园艺学院/海南省热带园艺作物品质调控重点实验室,海口570228 [2]海南大学热带作物学院/海南省耐盐作物生物技术重点实验室,海口570228

出　　处：《农业生物技术学报》2022年第7期1279-1289,共11页Journal of Agricultural Biotechnology

基　　金：国家重点研发计划(2018YFE0207203-2);海南省自然科学基金(318QN189);海南省教育厅项目(Hnky2021-19)。

摘　　要：盐胁迫是限制植物生长发育的主要非生物胁迫之一。本研究以盐生植物海马齿(Sesuvium portulacastrum)为研究对象,分析海马齿幼苗在400和800 mmol/L NaCl处理0、6、12、24、48和72 h后,其根和叶生理指标变化及相关耐盐基因的表达情况。结果表明:在400 mmol/L NaCl胁迫下,海马齿幼苗在胁迫初期出现萎蔫现象,随着胁迫时间的延长,海马齿幼苗的生长逐渐稳定;在800 mmol/L NaCl胁迫下,海马齿幼苗随着胁迫时间的延长出现萎蔫并最终死亡;叶和根中超氧化物歧化酶(superoxide dismutase,SOD)、抗坏血酸过氧化物酶(ascorbate peroxidase,APX)、过氧化物酶(peroxidase,POD)的活性在12 h内显著增加,可溶性糖和脯氨酸积累量增加较明显,此后酶活性和渗透调节物质含量均降低。相对电导率呈先升高后下降的趋势。400 mmol/L NaCl胁迫下,海马齿中耐盐相关基因质膜Na^(+)/H^(+)逆转运蛋白基因(salt overly sensitive 1,SOS1)、蛋白激酶基因(CBL-interaccting protein kinase 8,CIPK8)、类似钙调磷酸酶B亚基蛋白基因(calcineurin B-like 10,CBL10)与质膜H~+-ATPase基因(plasma membrane H^(+)-ATPase gene,AHA1)在根和叶中均呈上调表达,在800 mmol/L NaCl胁迫下,SpSOS1、SpCIPK8、SpCBL10和SpAHA1基因在海马齿叶中的表达量在处理后12 h达到最高,为同时间400 mmol/L NaCl胁迫下基因表达量的6.80、124.32、25.93和9.52倍。本研究为进一步研究植物在盐胁迫下生理指标及相关基因表达调控规律提供理论依据。Salt stress is one of the main abiotic stresses that restrict plant growth and development.In this study,the halophyte Sesuvium portulacastrum seedlings were treated with 400 and 800 mmol/L NaCl for 0,6,12,24,48 and 72 h,and the changes of physiological indexes in roots and leaves and the expression level of salt-related genes were analyzed.The results showed that Sesuvium portulacastrum seedlings withered at the beginning of stress,while the growth gradually stabilized with the extension of stress time when treated with400 mmol/L NaCl.While the seedlings wilted and died gradually with the extension of stress time under 800mmol/L NaCl.The activities of superoxide dismutase(SOD),ascorbate peroxidase(APX)and peroxidase(POD)in leaves and roots all increased significantly under 12 h treatment.The accumulation of soluble sugar and proline increased obviously,and then the enzyme activity and osmotic regulation substances contents decreased.The relative conductivity increased first and then decreased.Four salt-tolerant genes were selected for real-time fluorescence quantitative verification.The expression levels of salt-related genes,plasma membrane Na^(+)/H^(+) antiporter gene salt overly sensitive 1(SOS1),protein kinase gene CBL-interaccting protein kinase 8(CIPK8),Calcineurin B-like 10(CBL10)and plasma membrane H^(+)-ATPase gene(AHA1),were upregulated in the roots and leaves under salt stress.The expression levels of SpSOS1,SpCIPK8,SpCBL10 and SpAHA1 genes under 800 mmol/L NaCl reached the highest at 12 h after treatment,and the expressions were6.80,124.32,25.93 and 9.52 times of those under 400 mmol/L NaCl at the same time.This study provides a theoretical basis for the study of physiological indexes and related gene expression regulation of plants under salt stress.

关 键 词：海马齿 盐胁迫 生理变化 基因表达 

分 类 号：S-3[农业科学]