五种免疫增强剂对绍兴雏鸭胸腺组织部分炎症和凋亡相关因子表达的影响  

Effects of Five Immunopotentiators on the Expression of Some Inflammatory and Apoptosis-related Factors in Thymus Tissues of Shaoxing Ducklings(Anas platyrhynchos)

作　　者：顾天天 李国勤[2] 许文武 田勇 李柳萌[3] 陈斌丹 曾涛 陈黎 陶争荣[2] 沈军达 卢立志[1,2] GU Tian-Tian;LI Guo-Qin;XU Wen-Wu;TIAN Yong;LI Liu-Meng;CHEN Bin-Dan;ZENG Tao;CHEN Li;TAO Zheng-Rong;SHEN Jun-Da;LU Li-Zhi;无(State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products,Institute of Animal Science&Veterinary,Zhejiang Academy of Agricultural Science,Hangzhou 310021,China;Institute of Animal Husbandry and Veterinary Science,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,China;Zhejiang Guowei Technology Co.,Ltd.,Zhuji 311800,China)

机构地区：[1]浙江农业科学院省部共建农产品质量安全危害因子与风险防控国家重点实验室,杭州310021 [2]浙江省农业科学院畜牧兽医研究所,杭州310021 [3]浙江国伟科技有限公司,诸暨311800

出　　处：《农业生物技术学报》2022年第7期1339-1347,共9页Journal of Agricultural Biotechnology

基　　金：财政部和农业农村部:国家现代农业产业技术体系(CARS-42-6);浙江省农业(畜禽)新品种选育重大科技专项(2021C02068-10);浙江省重点研发项目(2018C04014)。

摘　　要：抗生素的过度使用严重威胁着鸭产业的发展,甚至危害人类健康,因此寻找可靠的抗生素替代物已成为当务之急。免疫增强剂可以通过提高自身非特异性免疫能力,增强机体应对病原微生物或病原体的防御。本研究旨在对5种不同的免疫增强剂对绍兴雏鸭(Anas platyrhynchos)胸腺组织凋亡及免疫因子相关基因表达的影响进行研究。将150只1日龄的绍兴雏鸭随机均分为6组,连续3 d分别于颈部皮下注射生理盐水、绿原酸、β-葡聚糖、黄芪黄酮、未甲基化CpG的核苷酸(CpG-containing DNA,CpG DNA)和鸡免疫球蛋白G(immunoglobulin G,IgG),18日龄采集胸腺组织,通过qPCR结合Western blot检测炎症相关基因和凋亡基因m RNA和蛋白表达水平。结果发现,与对照组相比,分别注射绿原酸、β-葡聚糖、黄芪黄酮、CpG-DNA和鸡IgG的绍兴雏鸭胸腺组织中,B淋巴细胞瘤2(B-cell lymphoma 2,Bcl2)mRNA和蛋白水平均显著降低(P<0.05),半胱天冬酶-3(Caspase3)的表达水平均在不同程度上调。此外,分别注射五种免疫增强剂后均在不同程度上提高了胸腺组织中诱生型一氧化氮合酶(inducible nitric oxide synthase,iNOS)和环氧化酶2(cyclooxygenase-2,COX2)的表达水平,干扰素-α(interferon-α,IFN-α)、干扰素-β(interferon-β,IFN-β)、白介素-1β(interleukin-1β,IL-1β)、肿瘤坏死因子α(tumor necrosis factor-α,TNF-α)、视黄酸诱导基因Ⅰ(retinoic acid-inducible gene-Ⅰ,RIG-Ⅰ)、Toll样受体3(toll-like receptor 3,TLR3)和Toll样受体7(toll-like receptor 7,TLR7)基因的mRNA水平均呈现不同程度的上调表达。综上所述,绿原酸、β-葡聚糖、黄芪黄酮、CpG-DNA和鸡IgG可作为免疫增强剂调控鸭先天性免疫。本研究为预防鸭重要传染性疾病提供新途径,同时也为动物生产过程中抗生素替代物的应用提供一定的参考。The misuse of antibiotics seriously imperils the development of duck husbandry and even endangers human health.Therefore,it is urgent to find reliable alternatives to antibiotics.Immunopotentiator can effectively increase the ability of specific immune responses to enhance the defense against microorganisms or pathogens.The aim of this study was to investigate the effects of 5 different immunopotentiators on the thymus apoptosis and immune-related gene expression in Shaoxing ducklings(Anas platyrhynchos).The 1501-day-old Shaoxing ducklings were randomly divided into 6 groups,and saline,chlorogenic acid,β-D-glucan,astragalus flavone,nonmethylated Cp G DNA and chicken immunoglobulin G(Ig G),were injected subcutaneously once daily for 3 d,respectively.At the age of 18 d,the m RNA and protein expression of apoptosis and immune-related genes were detected by q PCR and Western blot.The results showed that compared with control group,the m RNA and protein levels of thymus B-cell lymphoma 2(Bcl2)were significantly decreased during injecting chlorogenic acid,β-D-glucan,astragalus flavone,nonmethylated Cp G DNA and chicken Ig G,respectively(P<0.05).And the expression levels of thymus Caspase 3 showed significantly up-regulated in some extent(P<0.05).In addition,the expression level of thymus inducible nitric oxide synthase(i NOS)and cyclooxygenase-2(COX2)were increased in some extent during injecting 5 different immunopotentiators,respectively.Meanwhile,compared with control group,the levels of interferon-α(IFN-α),interferon-β(IFN-β),interleukin-1β(IL-1β),tumor necrosis factor-α(TNF-α),retinoic acid-inducible gene-I(RIG-I),toll-like receptor 3(TLR3)and toll-like receptor 7(TLR7)m RNA expression were up-regulated in some extent during injecting 5 different immunopotentiators,respectively.Taken together,chlorogenic acid,β-D-Glucan,astragalus flavone,nonmethylated Cp G DNA and chicken Ig G could be used as immunopotentiators to regulate duck innate immune.This study provides new insight to prevent the duck disease

关 键 词：免疫增强剂 胸腺 天然免疫应答 绍兴雏鸭 

分 类 号：S834[农业科学—畜牧学]