精胺对雌鹅空肠、回肠多胺代谢关键基因的表达及多胺水平的影响  

Effect of Spermine on Expression of Polyamine Metabolism-related Key Genes and Polyamine Contents in Jejunum and Ileum of Female Geese(Anser cygnoides)

作　　者：易治鑫 蒋易龙 李林祥 姜冬梅[1] 康波[1] YI Zhi-Xin;JIANG Yi-Long;LI Lin-Xiang;JIANG Dong-Mei;KANG Bo(College of Animal Science and Technology,Sichuan Agricultural University,Chengdu 611130,China;Bazhong Academy of Green Agriculture&Innovative Development,Bazhong 636000,China)

机构地区：[1]四川农业大学动物科技学院,成都611130 [2]巴中市绿色农业创新发展研究院,巴中636000

出　　处：《农业生物技术学报》2022年第8期1571-1579,共9页Journal of Agricultural Biotechnology

基　　金：国家自然科学基金(31201798);四川省教育厅青年基金(18ZB0469)。

摘　　要：精胺能促进肠道黏膜损伤修复,提高肠道消化酶活性,调节肠道多胺稳态和肠道功能。为研究精胺对鹅(Anser cygnoides)空肠和回肠多胺代谢关键基因表达及多胺水平的影响,用5和10 mg/kg精胺灌胃成年四川白鹅雌鹅,运用qPCR分析鹅空肠和回肠组织中多胺代谢关键基因表达量,高效液相色谱法检测空肠和回肠组织多胺含量。结果表明,5 mg/kg精胺处理组雌鹅空肠组织鸟氨酸脱羧酶抗酶抑制剂2(ornithine decarboxylase antizyme inhibitor 2,AZIN2)、鸟氨酸脱羧酶抗酶1(ornithine decarboxylase antizyme,OAZ1)和OAZ2基因表达量以及亚精胺和精胺含量显著高于对照组(P<0.05),而AZIN1和乙酰多胺氧化酶(acetylpolyamine oxidase,APAO)基因表达量显著低于对照组(P<0.05);5 mg/kg精胺处理组雌鹅回肠组织AZIN2和精胺合成酶(spermine synthase,SPMS)基因表达量显著高于对照组(P<0.05),而亚精胺/精胺N’-乙酰转移酶(spermidine/spermine-N’-acetyltransferase,SSAT)基因表达量显著降低(P<0.05);10mg/kg精胺组雌鹅空肠组织AZIN2、OAZ2、鸟氨酸脱羧酶(ornithine decarboxylase,ODC)、APAO和精胺氧化酶(spermine oxidase,SMO)表达量以及精胺含量显著高于对照组(P<0.05),而亚精胺合成酶(spermidine synthase,SPDS)表达量和腐胺含量显著降低(P<0.05);10 mg/kg精胺能显著上调雌鹅回肠AZIN2、ODC、SPDS和APAO表达(P<0.05),并显著降低腐胺含量(P<0.05)。本研究结果表明,外源性精胺可介导雌鹅小肠组织多胺代谢基因表达,参与调控小肠组织多胺稳态,且精胺对雌鹅空肠和回肠组织多胺稳态的影响程度存在一定差异,为研究鹅小肠多胺代谢功能提供了基础数据。Spermine accelerates the repair of intestinal mucosal injury, improves activities of intestinal digestive enzymes, polyamine homeostasis, and intestinal function. To research the effects of spermine on key genes related to polyamine metabolism and polyamine contents in the jejunum and ileum in Sichuan white goose(Anser cygnoides), the female geese were intragastrically administered with 5 and 10 mg/kg spermine(body weight). The mRNA expression levels of key genes related to polyamine metabolism and the content of polyamines in female geese in jejunum and ileum tissues were measured by qPCR and high performance liquid chromatography. The results showed that the expression levels of ornithine decarboxylase antizyme inhibitor 2 gene(AZIN2), ornithine decarboxylase antizyme 1(OAZ1) and OAZ2 genes, and the contents of spermidine and spermine in jejunum of female geese treated with spermine at 5 mg/kg were significantly higher than that in the control group(P<0.05), while expression levels of AZIN1 and acetylpolyamine oxidase gene(APAO) were significantly decreased(P<0.05). The expression levels of AZIN2 and spermine synthase gene(SPMS) in ileum of female geese treated with spermine at 5 mg/kg were significantly higher than that in the control group(P<0.05), while the mRNA expression level of spermidine/spermine-N’-acetyltransferase gene(SSAT) was significantly decreased(P<0.05). The expression levels of AZIN2, OAZ2, ornithine decarboxylase gene(ODC), APAO and spermine oxidase gene(SMO) and spermine content in jejunum of female geese treated with spermine at 10 mg/kg were significantly higher than that in the control group(P<0.05), while the m RNA expression level of spermidine synthase gene(SPDS) and putrescine content were significantly lower(P<0.05), respectively. The expression levels of AZIN2, ODC, SPDS and APAO in ileum of female geese treated with spermine at 10 mg/kg were significantly higher than that in the control group(P<0.05), while the content of putrescine was significantly decreased(P<0.05). These res

关 键 词：精胺 鹅 空肠 回肠 基因表达 

分 类 号：S835[农业科学—畜牧学]