拟南芥油体钙蛋白基因CALEOSIN3对胁迫环境及ABA诱导的响应  

Response of Arabidopsis Oil Body Protein Gene CALEOSIN3 to Stress Environment and ABA Induction

作　　者：向兰舟 胡娅晴 谢涵 刘春林[1,3] 阮颖[1,2] Xiang Lanzhou;Hu Yaqing;Xie Han;Liu Chunlin;Ruan Ying(Hunan Provincial Key Laboratory of Crop Epigenetie Regulation and Development,Hunan Agricultural University,Changsha,410128;College of Bioscience and Biotechnology,Hunan Agriculural University,Changsha,410128;Collge of Agricuture,Hunan Agriculural Univerily,Changsha,410128)

机构地区：[1]湖南农业大学,作物表观遗传调控与发育湖南省重点实验室,长沙410128 [2]湖南农业大学生物科学技术学院,长沙410128 [3]湖南农业大学农学院,长沙410128

出　　处：《分子植物育种》2022年第14期4685-4690,共6页Molecular Plant Breeding

基　　金：国家自然科学基金面上项目(31771838);湖南省教育厅科研项目(19K042)共同资助。

摘　　要：拟南芥CALEOSIN3(以下简称为AtCLO3)是一种油体膜蛋白,以钙结合蛋白的形式结合在油体表面,对维持油体结构及稳定具有重要的作用。为研究AtCLO3对胁迫环境的响应,我们将已构建的AtCLO3表达质粒pBI101-pCLO3∶∶GUS浸花法转化拟南芥,获得其转基因植株;利用GUS组织化学染色法和实时荧光定量PCR(RT-qPCR)技术分析正常生长条件下,以及高盐、高温胁迫和ABA诱导条件下的AtCLO3基因表达情况。结果显示,正常生长条件下,AtCLO3基因在成熟莲座叶(叶脉及叶缘排水器)、花和果荚外壳均有表达,其中在花器官中表达最为明显,在15 d幼苗中几乎无表达;根、茎和成熟种子中无表达;高盐、高温胁迫及ABA诱导条件下,AtCLO3基因表达均上调,其中高温胁迫下表达显著上调。结果表明,正常生长条件下,拟南芥AtCLO3基因主要在花器官中高表达,并对高温胁迫响应强烈,为进一步利用该基因用于作物抗逆性育种提供可行性依据。As an oil body membrane protein,Arabidopsis thaliana CALEOSIN3(hereinafter referred to as AtCLO3)plays an important role in maintaining the stability of oil body structure by binding to oil body surface in the form of calcium binding protein.In order to study the response of AtCLO3 to stress environment,we transformed the constructed AtCLO3 expression plasmid pBI101-pCLO3∶∶GUS into Arabidopsis thaliana to obtain transgenic plants.GUS histochemical staining and Quantitative Real-time PCR(RT-qPCR)were used to analyze the AtCLO3gene expression under normal growth conditions,high salt stress,high temperature stress and ABA induction.The results showed that under normal conditions AtCLO3 gene was expressed in leaves(leaf vein and leaf edge drainage apparatus),flower and pod shell.among which the expression was most obvious in flower organs,and there were extremely weak expression in 15 day seedlings;no expression was found in roots,stems and mature seeds;the expression of AtCLO3 gene was up-regulated under high salt,high temperature stress and ABA induction.Under high temperature stress,the gene upregulation was the highest.The above results indicated that AtCLO3 gene was highly expressed in flower organs under normal growth conditions,and it was highly responsive to high temperature stress.This study provides a feasible basis for further using this gene in crop stress resistance breeding.

关 键 词：拟南芥(Arabidopsis thaliana L.) AtCLO3 环境胁迫 ABA诱导 

分 类 号：Q943.2[生物学—植物学]