贮存和液体培养条件对越南油茶花粉生长的影响  

Effects of Storage and Liquid Culture Conditions on the Growth of Camellia vietnamensis Pollen

作　　者：马光耀 郑道君 孙秀秀[2] 夏腾飞 戚华沙 陈加利 王春梅[2] 王健 Ma Guangyao;Zheng Daojun;Sun Xiuxiu;Xia Tengfei;Qi Huasha;Chen Jiali;Wang Chunmei;Wang Jian(School of Horticulture,Hainan University,Haikou,571100;The Key Laboratory of Tropie Special Economic Plant Innovation and Uilization,Chengmai Research Stalion of National Germplasm Resources,Institule of Tropical Horticulure Research in Hainan Aeadery of Agricultural Sciencs,Haikou,571100;Sanya Academy of Hainan Academy of Agricultural Sciences,Sanya,S72000)

机构地区：[1]海南大学园艺学院,海口571100 [2]海南省农业科学院热带园艺研究所,国家作物种质资源澄迈观测实验站,海南省特种经济植物资源创新利用重点实验室,海口571100 [3]海南省农业科学院三亚研究院,三亚572000

出　　处：《分子植物育种》2022年第14期4858-4866,共9页Molecular Plant Breeding

基　　金：国家自然科学基金项目(31860082);海南省省属科研院所技术开发研究专项和农业农村部:物种品种资源保护项目(111821301354052033)共同资助。

摘　　要：为了分析不同贮存方式和液体培养条件对越南油茶花粉萌率发及花粉管伸长的影响,以及解析越南油茶花粉萌发特性的个体差异,本研究以越南油茶花粉为材料,采用离体培养法研究了培养基组分、培养温度、贮藏方式及不同单株对花粉生长的影响。结果表明:越南油茶花粉在含100 g/L蔗糖和0.05 g/L硼酸的液体培养基中,25℃条件下培养,萌发效果最好;不同个体花粉萌率发及花粉管伸长差异极显著;越南油茶花粉贮存最佳方式为干燥后置于-20℃贮存。本研究结果可为解析越南油茶“花而不实”引起低产问题和研发人工授粉技术提供参考依据。In order to analyze the effects of different storage methods and liquid culture conditions on pollen germination rate and pollen tube elongation of Camellia vietnamensis,and to analyze the individual differences in pollen germination characteristics of Camellia vietnamensis,this study uses the pollen of Camellia vietnamensis as the material,and uses the in vitro culture method to study the effects of medium components,culture temperature,storage methods and different individual plants on pollen growth.The results showed that the pollen of Camellia vietnamensis cultivated in liquid medium containing 100 g/L sucrose and 0.05 g/L boric acid at 25℃has the best germination effect.There are significant differences in pollen germination rate and pollen tube elongation among different individuals.The optimal storage method of pollen of Camellia vietnamensis is to store at-20℃after drying.The results of this study can provide a reference for analyzing the low yield problem caused by’flowering rather than fruiting’of Camellia vietnamensis and developing artificial pollination technology.

关 键 词：越南油茶 花粉生长 贮存 液体培养 

分 类 号：S794.4[农业科学—林木遗传育种]