不同保存条件对化学发光微粒子免疫分析法监测环孢素血药浓度的影响  

Effect of Different Preservation Conditions on Monitoring the Blood Concentration of Cyclosporine by Chemiluminescence Microparticle Immunoassay

作　　者：张凌鹏 陈井霞 杨云云 李丹 王学彬 高丽红 王卓 ZHANG Lingpeng;CHEN Jingxia;YANG Yunyun;LI Dan;WANG Xuebin;GAO Lihong;WANG Zhuo(Dept.of Pharmacy,the First Affiliated Hospital of Naval Medical University,Shanghai 200433,China)

机构地区：[1]海军军医大学第一附属医院药剂科,上海200433

出　　处：《中国医院用药评价与分析》2022年第8期900-903,共4页Evaluation and Analysis of Drug-use in Hospitals of China

基　　金：国家自然科学基金面上项目(No.82173900);促进市级医院临床技能与临床创新能力三年行动计划项目(No.SHDC2020CR4072);上海长海医院医学基础研究专项青年培育项目(No.2021JCQN19);上海长海医院青年启动基金项目(No.2020QNB11)。

摘　　要：目的:探讨全血标本不同保存条件对环孢素血药浓度测定结果的影响。方法:收集该院患者常规测定环孢素血药浓度后的废弃标本共计80例,每个标本混匀后分装3份,分别于室温(20~25℃)、冷藏(2~8℃)和冷冻(-35~-30℃)条件下避光保存7 d。采用化学发光微粒子免疫分析法测定不同保存条件下的环孢素血药浓度。结果:室温、冷冻保存7 d的环孢素浓度分别为(213.0±128.3)、(207.4±121.7)ng/mL,与采样当日测定的血药浓度(212.0±123.5)ng/mL相比,差异均无统计学意义(P=0.792、P=0.188);冷藏保存7 d的环孢素浓度为(198.4±113.8)ng/mL,较采样当日测定浓度低6.6%,差异有统计学意义(P=0.002)。结论:冷藏保存7 d的环孢素血药浓度普遍降低,室温或冷冻保存7 d对环孢素血药浓度无影响。若环孢素标本采样当日不能及时检测,可将标本于室温避光或冷冻保存,并于7 d内完成测定。OBJECTIVE:To probe into the effect of different preservation conditions on monitoring the blood concentration of cyclosporine in whole blood sample.METHODS:A total of 80 discarded samples for routine monitoring of blood concentration were collected from this hospital,each sample was mixed and evenly divided into 3 parts,which were preserved at room temperature(20-25℃),refrigerated(2-8℃)and frozen(-35--30℃)away from light for 7 days.Chemiluminescence microparticle immunoassay was adopted to detect the blood concentration of cyclosporine under different preservation conditions.RESULTS:The concentrations of cyclosporine were(213.0±128.3)ng/mL at room temperature and(207.4±121.7)ng/mL at frozen condition for 7 days,which had no statistically significant differences compared with the blood concentrations of(212.0±123.5)ng/mL measured on the sampled day(P=0.792,P=0.188);while the concentration of cyclosporine at refrigerated condition for 7 days was(198.4±113.8)ng/mL,which was 6.6%lower than that measured on the sampled day,with statistically significant difference(P=0.002).CONCLUSIONS:The blood concentration of cyclosporine is generally decreased after 7 days of refrigeration,while 7 days of preservation at room temperature or frozen have no effect on the blood concentration of cyclosporine.If the cyclosporine sample cannot be detected in time on the day of sampling,it can be preserved at room temperature or frozen away from light,and the determination should be completed within 7 days.

关 键 词：环孢素 治疗药物监测 保存条件 化学发光微粒子免疫检测法 

分 类 号：R979.5[医药卫生—药品]