当归四逆汤中细辛的药效成分及其毒性研究  

Research on the Pharmacodynamic Components and Its Toxicity of Asarum in Danggui Sini Decoction

作　　者：余玖霞 梅茜 许金国[2] 苏联麟 毛春芹[2] 谢辉[2] 陆兔林[2] YU Jiuxia;MEI Qian;XU Jinguo;SU Lianlin;MAO Chunqin;XIE Hui;LU Tulin(Dept.of Pharmacy,Nanjing Jiangning Hospital of Traditional Chinese Medicine,Nanjing 211100,China;College of Pharmacy,Nanjing University of Chinese Medicine,Nanjing 210046,China)

机构地区：[1]南京市江宁中医院药学部,南京211100 [2]南京中医药大学药学院,南京210046

出　　处：《中国医院用药评价与分析》2022年第8期915-919,共5页Evaluation and Analysis of Drug-use in Hospitals of China

基　　金：国家重点研发计划项目(No.2018YFC1707000)。

摘　　要：目的:研究当归四逆汤中细辛的药效成分及其毒性。方法:(1)建立细辛饮片水煎液及当归四逆汤的指纹图谱,采用中药色谱指纹图谱相似度评价系统软件进行分析。(2)急性毒性试验。取雄性ICR小鼠42只,随机均分为细辛单煎组、复方组。细辛单煎组小鼠以细辛单煎液最大浓度0.9784 g/mL,最大给药剂量0.5 mL/10 g灌胃给药;复方组小鼠以当归四逆汤最大浓度0.6228 g/mL,最大给药剂量0.5 mL/10 g灌胃给药,均1日2次,连续观察2周,记录受试小鼠活动、行为及死亡等情况,观察小鼠心、肝、脾、肺及肾等脏器和组织的体积、颜色和质地等变化,并拍照称重。结果:(1)建立了15批细辛饮片水煎液和23批当归四逆汤的指纹图谱,相似度均>0.90,并指认出7个共有成分,分别为1号峰、4号峰、19号峰、20号峰、23号峰、24号峰和25号峰,采用对照品指认出其中4号峰为阿魏酸,23号峰为细辛脂素。(2)急性毒性试验结果显示,两组小鼠2周内未见死亡;经大体解剖,肉眼见心、肝、脾、肺及肾等脏器和组织在体积、颜色和质地等方面无异常改变;复方组小鼠的最大给药量为31.14 g/kg,细辛单煎组小鼠的最大给药量为48.92 g/kg。结论:通过指纹图谱指认出当归四逆汤中包括阿魏酸、细辛脂素在内的7个细辛有效成分,为进一步研究当归四逆汤中细辛的作用机制提供了参考,并对其毒性进行了小鼠急性毒性试验,初步考察并验证了细辛及其复方用药的安全性。OBJECTIVE:To study the pharmacodynamic components and its toxicity of asarum in Danggui Sini decoction.METHODS:(1)The fingerprint spectrum of asarum water decoction and Danggui Sini decoction were established and analyzed by using similarity evaluation system of chromatographic fingerprint spectrum of traditional Chinese medicine.(2)Acute toxicity test.42 male ICR mice were taken and randomly divided into asarum single decoction group and compound group.The mice in the asarum single decoction group was administered by gavage with a maximum concentration of 0.9784 g/mL and a maximum dose of 0.5 mL/10 g of the asarum water decoction;while the mice in the compound group were administered by gavage with a maximum concentration of 0.6228 g/mL and a maximum dose of 0.5 mL/10 g of Danggui Sini decoction,both groups were administrated twice a day and observed for 2 weeks.To record the activity,behavior and death of the mice,to observe the changes of volume,color and texture of the heart,liver,spleen,lungs and kidneys of the mice,and take photos and weigh.RESULTS:(1)The fingerprint spectrum of 15 batches of asarum water decpctopm and 23 batches of Danggui Sini decoction were established,with the similarity was>0.90,and seven common components were identified,namely peak 1,peak 4,peak 19,peak 20,peak 23,peak 24 and peak 25,the reference substance was used to identify peak 4 as ferulic acid and peak 23 as asarinin.(2)The results of acute toxicity test showed that mice in both groups did not die within 2 weeks;the organs and tissues including heart,liver,spleen,lung and kidney showed no abnormal changes in volume,color and texture after gross autopsy;the maximum dose for mice in the compound group was 31.14 g/kg,and the maximum dose for mice in the asarum single decoction group was 48.92 g/kg.CONCLUSIONS:The fingerprint spectrum has identified seven active ingredients of asarum in Danggui Sini decoction,including ferulic acid and asarinin,which provides reference for further study of the mechanism of asarum in Danggui Sini d

关 键 词：细辛 当归四逆汤 指纹图谱 阿魏酸 细辛脂素 急性毒性试验 

分 类 号：R932[医药卫生—生药学]