Establishment of an efficient regeneration and genetic transformation system for Malus prunifolia Borkh. ‘Fupingqiuzi’  

作　　者：LIU Yu-song WANG Hong-ying ZHAO Yong-juan JIN Yi-bo LI Chao MA Feng-wang 

机构地区：[1]State Key Laboratory of Crop Stress Biology for Arid Areas/Shaanxi Key Laboratory of Apple,College of Horticulture,Northwest A&F University,Yangling 712100,P.R.China

出　　处：《Journal of Integrative Agriculture》2022年第9期2615-2627,共13页农业科学学报（英文版）

基　　金：supported by the National Key Research and Development Program of China(2019YFD1001403-4);the Key S&T Special Projects of Shaanxi Province,China(2020zdzx03-01-02);the earmarked fund for the China Agriculture Research System of MOF and MARA(CARS-27)。

摘　　要：Malus prunifolia Borkh. ‘Fupingqiuzi’ has significant ecological and economic value and plays a key role in germplasm development and resistance research. However, its long juvenile phase and high heterozygosity are barriers to the identification of ‘Fupingqiuzi’ progeny with excellent traits. In-vitro regeneration techniques and Agrobacterium-mediated genetic transformation systems can efficiently produce complete plants and thus enable studies of gene function.However, optimal regeneration and genetic transformation systems for ‘Fupingqiuzi’ have not yet been developed.Here, we evaluated the factors that affect the in-vitro regeneration and transformation of ‘Fupingqiuzi’. The best results were obtained when transverse leaf sections were used as explants, and they were grown in dark culture for three weeks with their adaxial sides contacting the culture medium(MS basal salts, 30 g Lsucrose, 8 g Lagar, 5 mg L6-benzylaminopurine(6-BA), 2 mg Lthidiazuron(TDZ), and 1 mg L1-naphthlcetic acid(NAA), pH 5.8). A genetic transformation system based on this regeneration system was optimized: after inoculation with A. tumefaciens solution for 8 min, 4 days of co-culture, and 3 days of delayed culture, the cultures were screened with cefotaxime(150 mg L) and kanamycin(15 mg L). We thus established an efficient regeneration and genetic transformation system for ‘Fupingqiuzi’, enabling the rapid production of transgenic material. These findings make a significant contribution to apple biology research.

关 键 词：Malus prunifolia Borkh.‘Fupingqiuzi’ regeneration system Agrobacterium-mediated transformation system 

分 类 号：S661.4[农业科学—果树学]