BcSDR1 is involved in regulation of glucose transport and cAMP and MAPK signaling pathways in Botrytis cinerea  

作　　者：SI He-long ZHANG Kang LI Bai YUAN Xue-mei ZANG Jin-ping CAO Hong-zhe XING Ji-hong DONG Jin-gao 

机构地区：[1]State Key Laboratory of North China Crop Improvement and Regulation,Hebei Agricultural University,Baoding 071000,P.R.China [2]Hebei Key Laboratory of Plant Physiology and Molecular Pathology,Hebei Agricultural University,Baoding 071000,P.R.China

出　　处：《Journal of Integrative Agriculture》2022年第9期2628-2640,共13页农业科学学报（英文版）

基　　金：supported by the National Natural Science Foundation of China(31972217 and 32072369);the Central Government Guides Local Science and Technology Development Projects,China(206Z6501G and 216Z6502G);the Research Project of Basic Scientific Research Business Fees in Provincial Universities of Hebei Province,China(KY2021043 and KY2021044)。

摘　　要：Botrytis cinerea is a typical necrotrophic pathogenic fungus that causes severe diseases in a wide range of plant species, leading to significant economic losses. Our previous study showed that BcSDR1 positively regulates growth,development, and pathogenicity of B. cinerea. However, the regulation mechanism of BcSDR1 and the relationship between BcSDR1 and cAMP and MAPK signaling pathways are not well understood. In this study, transcriptome data showed that BcSDR1 is involved in glucose transmembrane transport, signal transduction, secondary metabolism, and other biological processes. BcSDR1 mutant(BCt41) showed remarkably weak sensitivity to cAMP and MAPK signaling pathways specific inhibitors, SQ22536 and U0126, and significantly decreased cAMP content. The key genes of cAMP and MAPK signaling pathways, BcGB1, BcBTP1, BcBOS1, BcRAS1, and BcBMP3 were significantly upregulated,whereas BcPLC1, BcBCG1, BcCDC4, BcSAK1, BcATF1, and BcBAP1 were significantly downregulated(P<0.05).BcSDR1 was obviously upregulated in BcBCG2, BcBCG3, BcPKA1, and BcPKAR RNA interference(RNAi) mutants, but significantly downregulated in BcPKA2, BcBMP1, and BcBMP3 RNAi mutants. Thus, BcBCG2, BcBCG3, BcPKA1, and BcPKAR negatively regulate BcSDR1 expression, whereas BcPKA2, BcBMP1, and BcBMP3 positively regulate BcSDR1expression.

关 键 词：Botrytis cinerea BcSDR1 glucose transmembrane transport cAMP signaling pathway MAPK signaling pathway 

分 类 号：S432.4[农业科学—植物病理学]