Study on burrowing nematode, Radopholus similis, pathogenicity test system in tobacco as host  

作　　者：YANG Si-hua ZHAO Li-rong DING Sha TANG Shi-qiao CHEN Chun ZHANG Huan-xin XU Chun-ling XIE Hui 

机构地区：[1]Research Center of Nematodes of Plant Quarantine,Laboratory of Plant Nematology,College of Plant Protection,South China Agricultural University,Guangzhou 510642,P.R.China [2]Technology Center,Guangzhou Customs District,People's Republic of China,Guangzhou 510623,P.R.China [3]Comprehensive Technology and Service Center of Huizhou Port Customs,Huizhou 516080,P.R.China

出　　处：《Journal of Integrative Agriculture》2022年第9期2652-2664,共13页农业科学学报（英文版）

基　　金：supported by the Natural Science Foundation of Guangdong Province,China(2021A1515011273);the National Natural Science Foundation of China(31071665)。

摘　　要：Radopholus similis(Cobb 1893) Thorne(1949) is a destructive migratory endoparasitic plant nematode. In this study,the pathogenic process of R. similis infection in Nicotiana benthamiana(tobacco) was studied using quartz sand culture in laboratory. The results showed that R. similis mainly parasitised the root cortex, leading to cortical cell decomposition and tissue decay. We optimised the inoculation conditions to establish a method for determining the pathogenicity of R. similis as follows:(1) a glass culture tube was filled with quartz sand(about 1/3 of the height) and sterilised twice;(2) 20-day-old N. benthamiana seedlings were transplanted into test tubes and cultivated for 10 days at(25±1)°C;(3) R. similis female nematodes were inoculated in the root rhizosphere at a rate of 150 nematodes per plant;(4) the number of nematodes, disease severity, and growth of the plant at 30 days post-inoculation(dpi) were determined. The pathogenicity of eight R. similis populations from different hosts was determined, which proved the feasibility of this method.

关 键 词：Radopholus similis Nicotiana benthamiana POPULATIONS PATHOGENICITY 

分 类 号：S435.72[农业科学—农业昆虫与害虫防治]