脂质过氧化反应在纳米细菌损伤肾小管上皮细胞过程中的作用  

Effects of lipid peroxidation on HK-2 cells damaged by nanobacteria

作　　者：杨恒 钱彪[1,2] 刘志立 王勤章 郝志强[1,2] 王敬珅 汪渊 李永乐 谭明辉 郑丽英 YANG Heng;QIAN Biao;LIU Zhili;WANG Qinzhang;HAO Zhiqiang;WANG Jingshen;WANG Yuan;LI Yongle;TAN Minghui;ZHENG Liying(Department of Urology,The First Affiliated Hospital of Medical School of Shihezi University,Shihezi 832008;Department of Urology,The First Affiliated Hospital of Gannan Medical University,Ganzhou 341000,China)

机构地区：[1]石河子大学医学院第一附属医院泌尿外科,新疆石河子832008 [2]赣南医学院第一附属医院泌尿外科,江西赣州341000

出　　处：《现代泌尿外科杂志》2022年第8期691-694,共4页Journal of Modern Urology

基　　金：国家自然科学基金项目(No.82060135);赣南医学院重点课题项目(No.ZD201909);石河子大学课题(No.ZZZC201823A)。

摘　　要：目的探究脂质过氧化反应在纳米细菌(NB)损伤HK-2细胞过程中的作用。方法将NB组(NB菌液、胎牛血清、1640培养液+HK-2细胞)、四环素干扰组(四环素、NB菌液、胎牛血清、1640培养液+HK-2细胞)和空白对照组(胎牛血清、1640培养液+HK-2细胞)分别培养6、12、24 h,观察HK-2细胞的形态学变化。用超声粉碎HK-2细胞后检测Na^(+)/K^(+)ATP酶和Ca^(2+)/Mg^(2+)ATP酶活性,并检测培养液中乳酸脱氢酶(LDH)、过氧化氢(H_(2)O_(2))和丙二醛(MDA)的含量。结果HE结果可见,四环素干扰组中HK-2细胞的损伤程度明显低于NB组,干扰组细胞在共培养12、24 h时酶活性高于NB组(P<0.05)。在共培养12、24 h时,NB组培养液中H_(2)O_(2)和MDA的含量显著高于对照组(P<0.05),干扰组显著低于NB组(P<0.05)。结论NB损伤人肾小管上皮细胞HK-2的过程中,引起脂质过氧化反应,并且脂质过氧化反应的程度与人肾小管上皮细胞HK-2损伤程度成正相关。Objective To explore the effects of lipid peroxidation on human renal tubular epithelial HK-2 cells damaged by nanobacteria(NB).Methods HK-2 cells were divided into 3 groups:control group,NB group and tetracycline interference group.Hk-2 cells were co-cultured with each group for 6 h,12 h and 24 h,respectively.Morphological changes of HK-2 cells were observed with a light microscope.The activities of Na^(+)/K^(+)ATpase and Ca^(2+)/Mg^(2+)ATpase were detected after HK-2 cells were crushed with ultrasound.The contents of lactate dehydrogenase(LDH),hydrogen peroxide(H_(2)O_(2))and malondialdehyde(MDA)in the culture medium were determined.Results In NB group,tetracycline interference group and control group,the damage degree of HK-2 cells decreased successively,the enzyme activity increased successively,and the contents of H_(2)O_(2) and MDA decreased successively.Conclusion Lipid peroxidation is induced in the process of NB injury to HK-2 cells,and the degree of lipid peroxidation is positively correlated with the degree of injury.

关 键 词：肾结石 纳米细菌 脂质过氧化反应 HK-2细胞 四环素 

分 类 号：R692.4[医药卫生—泌尿科学]