小儿宝泰康颗粒对呼吸道病毒感染的抗病毒作用研究  

Antiviral activity of Xiaoer Baotaikang Granules against respiratory viruses in vitro

作　　者：刘军 杨光 沈梦欣 刘媛媛[1] 杨占秋[1] LIU Jun;YANG Guang;SHEN Mengxin;LIU Yuanyuan;YANG Zhanqiu(Institute of Medical Virology,School of Basic Medicine,Wuhan University,Wuhan 430071,China;Laboratory Department,Maternal and Child Health Hospital of Hubei Province,Wuhan 430070,China)

机构地区：[1]武汉大学基础医学院医学病毒学研究所,湖北武汉430071 [2]湖北省妇幼保健院检验科,湖北武汉430070

出　　处：《药学研究》2022年第8期491-495,544,共6页Journal of Pharmaceutical Research

基　　金：国家自然科学基金(No.30873104)。

摘　　要：目的观察小儿宝泰康颗粒对呼吸道合胞病毒和腺病毒感染细胞的抑制作用,以便为呼吸道病毒感染的治疗提供新的药物。方法通过细胞培养技术观察不同浓度的小儿宝泰康颗粒对HEp-2细胞的细胞毒作用,采用MTT法并结合细胞病变效应(CPE)法检测病毒感染细胞存活率和病毒抑制率,以利巴韦林作为阳性药物对照,评价该药物对呼吸道合胞病毒和腺病毒感染细胞的抗病毒活性。结果小儿宝泰康颗粒对HEp-2细胞的半数细胞毒性浓度(TC_(50))为4.4 mg·mL^(-1),对呼吸道合胞病毒的半数有效浓度(EC_(50))为2.03 mg·mL^(-1),而腺病毒的EC 50为0.65 mg·mL^(-1),抗病毒指数分别为2.17和6.75,在对两种病毒的直接灭活作用中,EC_(50)分别为4.13和8.99 mg·mL^(-1)。阳性对照药物利巴韦林在128 g·mL^(-1)时,对呼吸道合胞病毒和腺病毒的抑制率分别为92.7%和80.16%。结论小儿宝泰康颗粒是一种对呼吸道合胞病毒和腺病毒感染有抗病毒作用的药物,有临床应用前景和进一步研究的价值。Objective To investigate the antiviral activity of Xiaoer Baotaikang Granules on respiratory syncytial virus(RSV)and adenovirus(AdV)in vitro.Methods The cell viabilities of the Xiaoer Baotaikang Granules were determined by MTT assay on HEp-2 cells.Cytopathic effect(CPE)observation and MTT assay were applied to evaluate the antiviral activity of Xiaoer Baotaikang Granules and ribavirin was used as positive control drug.Results The TC_(50) value of Xiaoer Baotaikang Granules on HEp-2 cells was 4.4 mg·mL^(-1).Furthermore,Xiaoer Baotaikang Granules had inhibitory activity on viral replication in vitro with EC_(50) of 2.03 mg·mL^(-1) and 0.65 mg·mL^(-1) on RSV and AdV,resulting in TI of 2.17 and 6.75,respectively.In the virucidal activity,the ED_(50) of Xiaoer Baotaikang Granules against RSV and AdV was 4.13 and 8.99 mg·mL^(-1),respectively.Comparatively,inhibition rate was 92.7%and 80.16%against RSV and AdV in ribavirin control group.Conclusion Xiaoer Baotaikang Granules has antiviral activity against RSV and AdV on HEp-2 cells.

关 键 词：小儿宝泰康颗粒 呼吸道合胞病毒 腺病毒 抗病毒作用 细胞实验 

分 类 号：R285.5[医药卫生—中药学]