黄山松成熟胚培养与植株再生技术研究  

Research on mature embryo culture and plant regeneration of Pinus taiwanensis Hayata

作　　者：兰延钢 汤明霞[2] 许成芳[2] 王瑞佳 严涵薇 吴敏 项艳[1] LAN Yangang;TANG Mingxia;XU Chengfang;WANG Ruijia;YAN Hanwei;WU Min;XIANG Yan(School of Forestry and Landscape Architecture,Anhui Agricultural University,Hefei 230036;Landscape Bureau of Huangshan Scenic Area Management Committee,Huangshan 245899)

机构地区：[1]安徽农业大学林学与园林学院,合肥230036 [2]黄山风景区管理委员会园林局,黄山245899

出　　处：《安徽农业大学学报》2022年第3期368-373,共6页Journal of Anhui Agricultural University

基　　金：全球环境基金(GEF)赠款、联合国粮农组织(FAO)为国际执行机构、黄山风景区代表黄山市人民政府实施的“黄山地区生物多样性保护和可持续利用”项目资助。

摘　　要：以黄山松(Pinus taiwanensis Hayata)成熟胚为试验材料,进行离体培养与植株再生条件的初步研究。结果表明,黄山松成熟胚外植体表面灭菌以75%的乙醇处理1 min,3%的次氯酸钠处理10 min为适宜;愈伤组织诱导培养基以MS+1.0 mg·L^(-1)6-BA+0.2 mg·L^(-1) NAA为最佳;适宜的不定芽分化培养基为DCR+1.0 mg·L^(-1)6-BA+0.05 mg·L^(-1) NAA;不定芽伸长以DCR+0.1 mg·L^(-1)6-BA+0.05 mg·L^(-1) NAA为适宜;生根培养基以1/2 DCR+2.0 mg·L^(-1) IBA+0.05 mg·L^(-1) NAA为适宜。建立的植株再生体系为黄山松种质资源的保存、遗传改良及优质种苗繁殖等研究提供了参考。In this study,the mature embryo of Pinus taiwanensis Hayata was used as the experimental material to conduct a preliminary study of in vitro culture and plant regeneration conditions.The results showed that the suitable surface sterilization method for mature embryos of P.taiwanensis Hayata was 75%alcohol treatment for 1 min and 3%sodium hypochlorite for 10 min;the optimal callus induction medium was MS+1.0 mg·L^(-1)6-BA+0.2 mg·L^(-1) NAA;the optimal induction medium for adventitious buds was DCR+1.0 mg·L^(-1)6-BA+0.05 mg·L^(-1) NAA;the suitable induction condition for adventitious bud elongation was DCR+0.1 mg·L^(-1)6-BA+0.05 mg·L^(-1) NAA;the optimal medium for rooting was 1/2 DCR+2.0 mg·L^(-1) IBA+0.05 mg·L^(-1) NAA.The results obtained in this study will be of great significance for the preservation,genetic improvement and high-quality seedling reproduction of P.taiwanensis Hayata germplasms.

关 键 词：黄山松 成熟胚 离体培养 植株再生 

分 类 号：S791.24[农业科学—林木遗传育种]