脂多糖刺激干扰素调节因子3促进小鼠腹腔巨噬细胞白介素-17表达  

Interferon regulatory factor 3 promotes the expression of interleukin-17 in mouse peritoneal macrophages stimulated by lipopolysaccharide

作　　者：李婷 黄小园 马凤 廖承浩 王凯[3] Li Ting;Huang Xiaoyuan;Ma Feng;Liao Chenghao;Wang Kai(School of Basic Medical Sciences and Forensic Medicine,Hangzhou Medical College,Hangzhou 311399;The Affiliated Hospital of Stomatology,School of Stomatology,Zhejiang University School of Medicine,and Key Laboratory of Oral Biomedical Research of Zhejiang Province,Hangzhou 310006;2nd Affiliated Hospital,School of Medicine,Zhejiang University,Hangzhou,310009 China)

机构地区：[1]杭州医学院基础医学与法医学院,杭州311399 [2]浙江大学医学院附属口腔医院,浙江大学口腔医学院,浙江省口腔生物医学研究重点实验室,杭州310006 [3]浙江大学医学院附属第二医院,杭州310009

出　　处：《解剖学杂志》2022年第3期219-221,278,共4页Chinese Journal of Anatomy

摘　　要：目的:探讨干扰素调节因子3(IRF3)对脂多糖(LPS)刺激小鼠腹腔巨噬细胞促进白细胞介素-17(IL-17)表达的初步作用机制。方法:小鼠腹腔注射3%巯基乙酸肉汤,3 d后提取C57BL/6野生和IRF3基因敲除小鼠的腹腔巨噬细胞,培养过夜后添加LPS。收集细胞培养上清液,酶联免疫吸附试验检测细胞因子IL-17和白细胞介素-6(IL-6)的表达;提取细胞蛋白质,免疫印迹检测细胞核因子κB抑制蛋白(IκB)α、IRF3、磷酸化信号转导子和转录激活子3(STAT3)的蛋白水平。结果:LPS刺激小鼠腹腔巨噬细胞后,IRF3可显著地促进IL-17的产生,同时伴随着IL-6的产生、IκBα蛋白的降解及STAT3的磷酸化。结论:IRF3可能通过促进IL-6的产生,间接地激活STAT3的磷酸化,进而促进IL-17的产生。Objective:To explore the effect of interferon regulatory factor 3(IRF3)on the expression of interleukin-17(IL-17)in mouse peritoneal macrophages stimulated by lipopolysaccharide(LPS).Methods:Mice were intraperitoneally injected with 3%thioglycolic acid broth.Three days later,the peritoneal macrophages of C57BL/6 wild and IRF3 gene knockout mice were extracted and cultured overnight,then stimulated with LPS.Enzyme linked immunosorbent assay was used to detect the expression of cytokines IL-17 and interleukin-6(IL-6)in cell culture supernatant.Western blotting was used to detect the expression of inhibitor of nuclear factor kappa B(IκB)α,IRF3,phosphorylated signal transducer and transcription activator 3(STAT 3)in cells.Results:After LPS stimulation,the expression of IL-17 in mouse peritoneal macrophages was significantly promoted by IRF3,accompanied by the production of IL-6,the degradation of IκBα,and STAT3 phosphorylation.Conclusions:IRF3 may indirectly activate STAT3 phosphorylation by promoting the production of IL-6,thereby promoting the production of IL-17.

关 键 词：巨噬细胞 干扰素调节因子3 白细胞介素-17 白细胞介素-6 信号转导子和转录激活子3 脂多糖 

分 类 号：R392[医药卫生—免疫学]