ECM/SVF-gel对比Coleman脂肪治疗糖尿病大鼠创面的效果与机制研究  

Study on effect and mechanism of ECM/SVF-gel compared with Coleman fat in treatment of wound of diabetic rats

作　　者：赵文懿 杨中玉 孙晓涵 宋培军[1] 徐静[1] ZHAO Wenyi;YANG Zhongyu;SUN Xiaohan;SONG Peijun;XU Jing(Department of Plastic Surgery,First Affiliated Hospital of Bengbu Medical College,Bengbu,Anhui 233000,China;Tissue Transplantation Laboratory,Bengbu Medical College,Bengbu,Anhui 233000,China)

机构地区：[1]蚌埠医学院第一附属医院整形外科,安徽蚌埠233000 [2]蚌埠医学院组织移植实验室,安徽蚌埠233000

出　　处：《重庆医学》2022年第16期2719-2722,2729,共5页Chongqing medicine

摘　　要：目的对脂肪来源干细胞基质胶(ECM/SVF-gel)与Coleman脂肪治疗糖尿病大鼠创面的疗效进行对比,并探讨ECM/SVF-gel促进创面愈合的作用机制。方法选取体重250~300 g的SD雄性大鼠10只,腹腔注射链脲佐菌素进行糖尿病造模,成功后以直径10 mm的组织活检器于大鼠背部制备2个全层皮肤缺损创面,将预先制备好的同种异体Coleman-条件培养基(CM)和ECM/SVF-gel-CM分别注射于2个创面,每个创面各注射100μL,分别命名A组、B组。记录各创面术后第3、7、10、14天愈合情况并计算对比创面愈合率,并在术后第7、14天取创面皮肤组织进行CD34免疫组织化学染色,运用酶联免疫吸附试验试剂盒检测并对比Coleman-CM和ECM/SVF-gel-CM生长因子——表皮细胞生长因子(EGF)、碱性成纤维生长因子(bFGF)、血管内皮生长因子(VEGF)和转化生长因子(TGF-β1)表达水平。结果与A组创面比较,B组创面各时间点愈合情况均更好,创面愈合率更高,差异有统计学意义(P<0.05),新生血管更多;B组ECM/SVF-gel-CM生长因子——EGF、bFGF、VEGF、TGF-β1表达水平均高于A组,其中EGF、bFGF、VEGF表达水平比较,差异均有统计学意义(P<0.05)。结论与Coleman脂肪比较,ECM/SVF-gel促进糖尿病大鼠创面愈合的效果更优,其作用机制可能为通过旁分泌途径分泌生长因子作用于创面。Objective To compare the effect of ECM/SVF-gel and Coleman fat in the treatment of diabetic rat wound,and to explore the mechanism of ECM/SVF-gel in promoting the wound healing.Methods Ten SD male rats with body weight of 250-300 g were selected,after successfully constructing the diabetic molding by intraperitoneal injection of STZ,two full-layer skin defect wounds were made in the back of the rats by the tissue biopsy instrument with a diameter of 10 mm,and the pre-prepared allogeneic Coleman-CM and ECM/SVF-gel-CM were injected in two wounds respectively,100μL at each wound.On postoperative 3,7,10,14 d,the healing situation of each wound was recorded respectively,the wound healing rate was calculated and compared,and the wound skin tissue was taken on postoperative 7,14 d for conducting the CD34 immunohistochemical staining,and the ELISA kit was used to detect and compare the expression levels of growth factors EGF,bFGF,VEGF,and TGF-β1 in Coleman-CM and ECM/SVF-gel-CM between Coleman-CM and ECM/SVF-gel-CM.Results Compared with the wounds injected with Coleman-CM,the healing situation of wounds injected with ECM/SVF-gel-CM was better at each time point,the wound healing rate was higher,and the differences were statistically significant(P<0.05),and there were more neovascularization.The expression levels of growth factors EGF,bFGF,VEGF and TGF-β1 in ECM/SVF-gel-CM were higher than those in Coleman-CM,in which the expression levels of EGF,bFGF and VEGF had statistically significant difference(P<0.05).Conclusion Compared with Coleman fat,the effect of ECM/SVF-gel for promoting wound healing in diabetic rats is more superior,and its mechanism may secret the growth factors to act on the wound through paracrine pathway.

关 键 词：脂肪来源干细胞基质胶 科尔曼脂肪 糖尿病 创面 动物实验 

分 类 号：R62[医药卫生—整形外科]