miR-615-5p在高血压患者血清中表达及其调控Akt/eNOS信号通路的机制  

Expression of microRNA-615-5p in serum of patients with hypertension and its regulation of Akt/eNOS signaling pathway

作　　者：娄满[1] 高春燕[1] 王宏业 LOU Man;GAO Chun-Yan;WANG Hong-Ye(Department of Geriatrics,Harrison International Peace Hospital,Hengshui,053000,Hebei,China)

机构地区：[1]哈励逊国际和平医院老年病一科,河北衡水053000 [2]哈励逊国际和平医院中医科,河北衡水053000

出　　处：《中国老年学杂志》2022年第17期4279-4284,共6页Chinese Journal of Gerontology

基　　金：河北省中医药管理局科技计划项目(2019356);衡水市科技计划项目(2016014091Z)。

摘　　要：目的探讨miR-615-5p在高血压患者血清中表达及其潜在的调控机制。方法100例患有至少10年高血压患者为观察组和100例非高血压患者(骨质疏松18例、前列腺增生10例、慢性肠炎15例、胃食管反流12例、甲状腺结节23例、轻度贫血8例、胃炎胃溃疡14例)为正常组。运用定量实时逆转录聚合酶链反应(qRT-PCR)检测mRNA表达。HMEC-1细胞用于体外模型,再转染miR-615-5p和simiR-615-5p调控miR-615-5p表达和细胞活性。结果与正常组比较,miR-615-5p在高血压患者血清中的表达被上调,miR-615-5p与右心室收缩压(RVSP)及右心室肥厚指数RV/(LV+S):正相关。上调miR-615-5p抑制血管内皮细胞增殖,增加5-乙炔基-2′脱氧尿嘧啶核苷(Edu)细胞比例。下调miR-615-5p促进血管内皮细胞增殖,减少Edu细胞比例。上调miR-615-5p激活caspase-3/9活性表达水平,下调miR-615-5p抑制caspase-3/9活性表达水平。蛋白激酶B(Akt)是miR-615-5p调节血管内皮细胞的靶点,上调miR-615-5p抑制p-Akt和p-内皮型一氧化氮合酶(eNOS)蛋白表达量。Akt激动剂抑制miR-615-5p调节血管内皮细胞的作用。结论miR-615-5p在高血压患者血清中的表达上调,miR-615-5p通过抑制Akt/eNOS信号通路,阻止血管内皮细胞增殖,提示miR-615-5p可作为预测高血压的良好分子标志物。Objective To investigate the expression of miR-615-5p in the serum of hypertension patients and its potential regulatory mechanism.Methods 100 patients with hypertension for at least 10 years were included in the observation group and 100 patients without hypertension were included in the normal group(there were 18 cases of osteoporosis,10 cases of prostatic hyperplasia,15 cases of chronic enteritis,12 cases of gastroesophageal reflux,23 cases of thyroid nodules,8 cases of mild anemia,and 14 cases of gastritis and gastric ulcer).Quantitative real-time reverse transcription polymerase chain reaction(QRT-PCR)was used to detect mRNA expression.HMEC-1 cells were used in vitro model to transfect with miRNA-615-5p and simiRNA-615-5p to regulate miR-615-5p expression and cell activity.Results Compared with the normal group,the expression of miR-615-5p in the serum of observation group was up-regulated,and there was a positive correlation between miR-615-5p and the right ventricular systolic pressure(RVSP),the right ventricular hypertrophy index RV/(LV+S).Up-regulation of miR-615-5p inhibited the proliferation of vascular endothelial cells and increased the proportion of 5-ethynyl-2′deoxyuracil nucleoside(Edu)cells.Down-regulation of miR-615-5p promoted the proliferation of vascular endothelial cells and reduced the proportion of Edu cells.Up-regulated expression level of caspase-3/9 activity was activated by miR-615-5p,down-regulated the expression level of caspase-3/9 activity was inhibited by miR-615-5p.Protein kinase B(Akt)was the target of miR-615-5p in regulating vascular endothelial cells,and up-regulation of miR-615-5P inhibited the expression of p-Akt and p-endothelial nitric oxide synthase(eNOS)proteins.Akt agonists inhibited the regulation of miR-615-5p on vascular endothelial cells.Conclusions miR-615-5p could be up-regulated in the serum of patients with hypertension.By inhibiting Akt/eNOS signal pathway,miR-615-5p could prevent the proliferation of vascular endothelial cells,suggesting that miR-615-5p cou

关 键 词：高血压 miR-615-5p 蛋白激酶B(Akt) 内皮型一氧化氮合酶(eNOS) 

分 类 号：R54[医药卫生—心血管疾病]