何首乌中大黄素-大黄素二蒽酮抗心肌缺血作用研究  

Effect of Emodin-emodin Dianthrones from Polygoni Multiflori Radix on Myocardial Ischemia

作　　者：杨建波[1] 汪祺[1] 王莹[1] 程显隆[1] 魏锋[1] 陈子涵 孙华[2] 高慧宇 王雪婷 宋云飞 马双成[1] YANG Jian-bo;WANG Qi;WANG Ying;CHENG Xian-long;WEI Feng;CHEN Zi-han;SUN Hua;GAO Hui-yu;WANG Xue-ting;SONG Yun-fei;MA Shuang-cheng(National Institutes for Food and Drug Control,Beijing 100050,China;Institute of Materia Medica,Chinese Academy of Medical Sciences,Beijing 100050,China)

机构地区：[1]中国食品药品检定研究院,北京100050 [2]中国医学科学院药物研究所,北京100050

出　　处：《中国现代中药》2022年第8期1420-1424,共5页Modern Chinese Medicine

基　　金：国家自然科学基金项目(81973476)。

摘　　要：目的:研究何首乌Polygonum multiflorum Thunb.干燥块根中大黄素-大黄素二蒽酮的抗心肌缺血作用。方法:采用大孔吸附树脂、反相硅胶和制备型高效液相色谱等柱色谱方法快速制备大黄素-大黄素二蒽酮。采用雄性昆明种小鼠制备异丙肾上腺素诱导的心肌缺血模型,灌胃给予大黄素-大黄素二蒽酮(10 mg·kg–1),以地尔硫卓为阳性对照组,观察其对小鼠的保护作用。采用心电图检测仪监测小鼠心电图ST段抬高情况。采用酶联免疫吸附测定法(ELISA)试剂盒检测小鼠血清中心肌损伤生物标记物心肌肌钙蛋白-T (cTn-T)、肌酸激酶同工酶(CK-MB)水平。结果:何首乌中分离得到的大黄素-大黄素二蒽酮在10 mg·kg–1剂量下能显著改善心肌缺血小鼠心电图ST段抬高情况,降低血清cTn-T、CK-MB水平,改善心肌缺血损伤状态。结论:何首乌中大黄素-大黄素二蒽酮显示较好的抗心肌缺血作用,值得深入研究。Objective: To explore the effect of emodin-emodin dianthrones isolated from Polygoni Multiflori Radix(PMR) on myocardial ischemia. Methods: The emodin-emodin dianthrones were isolated by macroporous adsorption resin,reversed-phase silica, and preparative high performance liquid chromatography. Myocardial ischemia was induced in male Kunming mice with isoprenaline, and then the model mice were given(i.g.) the emodin-emodin dianthrones(10 mg·kg–1)and diltiazem was used in the positive control group. Then the effect on the disease was observed. Electrocardiogram detector was used to monitor the elevation of ST elevations on the electrocardiogram in mice. Enzyme-linked immunosorbent assay(ELISA) kit was used to detect the levels of cardiac troponin T(cTn-T) and creatine kinase isoenzyme(CK-MB), biomarkers of myocardial injury in serum of mice. Results: The emodin-emodin dianthrones significantly improved the ST elevations on the electrocardiogram, decreased the level of serum myocardial damage biomarker cTn-T and the level of myocardial enzyme, and alleviated myocardial ischemia in mice. Conclusion: The emodin-emodin dianthrones are effective against myocardial ischemia, which are worthy of further research.

关 键 词：何首乌 分离与纯化 大黄素-大黄素二蒽酮 抗心肌缺血 

分 类 号：R284[医药卫生—中药学] R285[医药卫生—中医学]