河南省牛病毒性腹泻病毒分子流行病学调查与分析  

Investigation and Analysis of Molecular Epidemiology of Bovine Viral Diarrhea Virus in Henan Province

作　　者：杨德新 徐峥嵘[2] 王新庄[1] 闫磊 朱小洁 薛永康 刘晓曼 张震 YANG De-xin;XU Zheng-rong;WANG Xin-zhuang;YAN Lei;ZHU Xiao-jie;XUE Yong-kang;LIU Xiao-man;ZHANG Zhen(College of Veterinary Medicine,Henan Agricultural University,Zhengzhou,Henan,450002,China;Animal Disease Prevention and Control Center of Gansu Province,Lanzhou,Gansu,730046,China;Henan Dairy Herd Improvement Center,Zhengzhou,Henan,450046,China;Henan International Union Laboratory of Dairy Herd Healthy Breeding,Zhengzhou,Henan,450046,China;Henan Breeding Cattle Genetic Properties Testing Center,Zhengzhou,Henan,450046,China;College of Animal Science and Technology,College of Veterinary Medicine,Huazhong Agricultural University,Wuhan,Hubei,430070,China)

机构地区：[1]河南农业大学动物医学院,河南郑州450002 [2]甘肃省动物疫病预防控制中心,甘肃兰州730046 [3]河南省奶牛生产性能测定中心,河南郑州450046 [4]河南省奶牛健康养殖国际联合实验室,河南郑州450046 [5]河南省种牛遗传性能测定中心,河南郑州450046 [6]华中农业大学动物科技学院动物医学院,湖北武汉430070

出　　处：《动物医学进展》2022年第8期126-130,共5页Progress In Veterinary Medicine

基　　金：河南省科技攻关项目(2021年-2022年);河南省奶牛呼吸疾病综合征发病规律及其主要致病菌潜在公共危害研究(212102110371);基于牛奶光谱的奶牛乳房炎快速批量早期诊断新技术的研发(212102110011)。

摘　　要：为了解河南地区规模化奶牛场牛病毒性腹泻病毒(Bovine viral diarrhea virus, BVDV)的感染情况及主要基因型,用商品化酶联免疫吸附试验(ELISA)试剂盒对来自河南省14个市和不同地区的40个规模化奶牛养殖场共5 486份血清中BVDV抗原进行检测,同时对ELISA方法检测的97份抗原阳性样本,用套式逆转录-聚合酶链式反应(nRT-PCR)进行BVDV抗原检测和基因分型。结果表明,A10奶牛场BVDV抗原阳性率最高,为7.8%(11/141),A11、A17奶牛场BVDV抗原阳性率最低,为0.66%(1/151)。不同牛场的BVDV抗原阳性率为0~7.8%,平均阳性率为1.77%;河南地区牛病毒性腹泻病毒的基因型均为BVDV-1型,未发现其他基因型。提示BVDV在河南省规模化奶牛场存在,但不同地区、不同牛场的BVDV阳性率不同,主要以BVDV-1型感染为主。In order to understand the infection status and main genotypes of bovine viral diarrhea virus(BVDV) in large-scale dairy farms in Henan, commercialized enzyme-linked immunosorbent assay(ELISA) was used to detect BVDV antigen in a total of 5486 sera from 40 large-scale dairy farms in 14 cities and different regions of Henan province, 97 antigen-positive samples detected by ELISA method and gene detection using nested reverse transcription polymerase chain reaction(nRT-PCR).The results showed that: A10 dairy farms, the highest BVDV antigen positive rate was 7.8%(11/141),A11,A17 dairy farms, the lowest BVDV antigen positive rate was 0.66%(1/151).The positive rates of BVDV antigen in different cattle farms were 0-7.8%,and the average positive rate was 1.77%;the genotypes of BVDV in Henan area were all BVDV-1,and no others were found.The results of the study suggest that BVDV exists in large-scale dairy farms in Henan province, but there are differences in the positive rates of BVDV in different regions and cattle farms, mainly based BVDV-1 infection.

关 键 词：牛病毒性腹泻病毒 流行病学 酶联免疫吸附试验 套式逆转录-聚合酶链式反应 抗原 

分 类 号：S852.653[农业科学—基础兽医学]