牛结节性皮肤病病毒在BHK-21细胞中的生长特性分析  

Analysis of Growth Characteristics of Lumpy Skin Disease Virus in BHK-21 Cells

作　　者：马春玲 蔺玉刚 杨雪 龚真莉[3] 岳亚辉 任善会 王相伟 杨增岐[4] 殷相平[3] 孙跃峰[3] 陈豪泰[3] 万学瑞[1] MA Chun-ling;LIN Yu-gang;YANG Xue;GONG Zhen-li;YUE Ya-hui;REN Shan-hui;WANG Xiang-wei;YANG Zeng-qi;YIN Xiang-ping;SUN Yue-feng;CHEN Hao-tai;WAN Xue-rui(Gansu Agricultural University,Lanzhou,Gansu,730070,China;Tarim Univerinary,Xinjiang,Alar,843300,China;Key Laboratory of Veterinary Etiological Biology,Lanzhou Veterinary Research Institute,CAAS,Gansu,Lanzhou,730016,China;Northwest Agriculture and Forestry University,Yangling,Shaanxi,712100,China)

机构地区：[1]甘肃农业大学,甘肃兰州730070 [2]新疆塔里木大学,新疆阿拉尔843300 [3]中国农业科学院兰州兽医研究所,甘肃兰州730016 [4]西北农林科技大学,陕西杨凌712100

出　　处：《动物医学进展》2022年第9期7-12,共6页Progress In Veterinary Medicine

基　　金：甘肃农业大学兽医学省级特色学科开放课题(GSAU-XKJS-2018-071);中国农业科学院兰州兽医研究所基本科研业务费(1610312021008)。

摘　　要：为探究国内流行的牛结节性皮肤病病毒(Lumpy skin disease virus,LSDV)野毒株在鼠源BHK-21细胞中的复制生长特性,用间接免疫荧光、蛋白免疫印迹和透射电镜方法,确定了在BHK-21细胞系中能够感染中国流行的LSDV/FJ/CHA/2021毒株。蛋白免疫印迹和病毒生长曲线结果均表明,LSDV野毒株呈现时间依赖性的方式在BHK-21细胞中进行有效复制。电镜结果证明LSDV病毒工厂位于BHK-21细胞质内而非细胞核内。结果表明,LSDV国内流行毒株能够在BHK-21细胞中进行高效复制,为探究LSDV感染的致病机制、动物模型建立及弱毒疫苗的研制奠定重要理论基础。This study aimed to explore the replication and growth characteristics of the domestic epidemic lumpy skin disease virus(LSDV)wild-type strain in hamster origin BHK-21 cells.In this study,the LSDV/FJ/CHA/2021 strain,which is epidemic in China,can infect and replicate in the BHK-21 cell line was identified by indirect immunofluorescence,Western blot,and transmission electron microscopy methods.The Western blot and one-step growth curve results showed that LSDV wild strain could replicate effectively in BHK-21 cells in a time-dependent manner.The results of the transmission electron microscope further proved that the viral factory of LSDV is located in the cytoplasm of BHK-21 rather than in the nucleus.Our results showed that the domestic epidemic strain of LSDV can replicate efficiently in BHK-21 cells,which plays a vital theoretical foundation for further exploring the infection and pathogenesis mechanism of LSDV,establishing animal models,and developing attenuated vaccines.

关 键 词：牛结节性皮肤病病毒 BHK-21细胞 病毒增殖 生长特性 

分 类 号：S857.1[农业科学—临床兽医学]