鹅源福氏志贺氏菌分离鉴定及耐药性分析  

Isolation,Identification and Dug Rsistance Analysis of Shigella flexneri Isolated from Geese

作　　者：路振香[1,2] 王东 洪皓正 王浩 王奇 周明 郭伟娜 刘畅[1,2] 张宁 李贺侠[4] LU Zhen-xiang;WANG Dong;HONG Hao-zheng;WANG Hao;WANG Qi;ZHOU Ming;GUO Wei-na;LIU Chang;ZHANG Ning;LI He-xia(College of Animal Science,Anhui Sciences and Technology University,Fengyang,Anhui,233100,China;Anhui Province Key Laboratory of Animal Nutritional Regulation and Health,Anhui Science and Technology University,Fengyang,Anhui,233100,China;Qixia District Animal Health Authority,Nanjing,Jiangsu,210046,China;Hexian Animal Disease Prevention and Control Center,Hexian,Anhui,238200,China)

机构地区：[1]安徽科技学院动物科学学院,安徽凤阳233100 [2]动物营养调控与健康安徽省重点实验室,安徽凤阳233100 [3]南京市栖霞区动物卫生监督所,江苏南京210046 [4]安徽省和县动物疫病预防与控制中心,安徽和县238200

出　　处：《动物医学进展》2022年第9期53-56,共4页Progress In Veterinary Medicine

基　　金：安徽科技学院校级发展基金项目(FZ220140);安徽科技学院大学生创新创业训练计划项目(S202010879118);安徽省高校协同创新项目(GXXT-2019-035)。

摘　　要：对某养殖户送检的腹泻病死鹅进行病原分离鉴定、耐药性和致病性分析。结果分离到1株革兰氏阴性细小杆菌,该菌16S rRNA的序列与GenBank上登录号为NR-026331.1福氏志贺氏菌的同源性为99.38%,确定该菌为福氏志贺氏菌。该菌对头孢西丁、头孢曲松、头孢噻呋钠等敏感。该菌含有卡那霉素类耐药基因aadA1、β-内酰胺酶类耐药基因blatem-1以及磺胺类耐药基因Sul1,并含有编码调控蛋白的基因vira、编码黏附与侵入肠黏膜细胞肠毒性因子基因Ia1以及编码志贺氏菌肠毒素1基因set1B。The pathogenic isolation and identification,drug resistance and pathogenicity analysis were carried out on the dead geese caused by enteritis and diarrhea.Results showed that a Gram-negative tiny bacterial strain was isolated.The sequence of 16S rRNA was 99.38%identical with that of NR-026331.1 Shigella flexneri in GenBank.Therefore,it was identified as Shigella flexneri.The strain was sensitive to cefoxitin,ceftriaxone and ceftiofur sodium.It contains the kanamycin-resistant gene aadA1,theβ-lactam enzyme-resistant gene blatem-1 and sulfonamide-resistant gene Sul1.The bacterium contains vira,which codes for multiple regulatory protein genes,Ia1,which codes for adhesion and invasion of enterocyte enterotoxin gene,and set1B,which codes for Shigella enterotoxin 1 gene.

关 键 词：福氏志贺氏菌 分离鉴定 耐药基因 毒力基因 鹅 

分 类 号：S852.61[农业科学—基础兽医学]