不同百合种质资源对灰霉病的抗性  

Resistance of different lily germplasm resources to Botrytis cinerea

作　　者：张铭芳 李卉[2] 韩东洋 杜运鹏[1] 薛静[1] 高俊莲[1] 张秀海[1] ZHANG Ming-fang;LI Hui;HAN Dong-yang;DU Yun-peng;XUE Jing;GAO Jun-lian;ZHANG Xiu-hai(Institute of Grassland,Flowers and Ecology,Beijing Academy of Agriculture and Forestry Sciences,Key Laboratory of Urban Agriculture(North China),Ministry of Agriculture and Rural Affairs,Beijing 100097,China;School of Landscape Architecture,Beijing Forestry University,Beijing 100083,China)

机构地区：[1]北京市农林科学院草业花卉与景观生态研究所,农业农村部华北都市农业重点实验室,北京100097 [2]北京林业大学园林学院,北京100083

出　　处：《江苏农业学报》2022年第4期1078-1084,共7页Jiangsu Journal of Agricultural Sciences

基　　金：北京市农林科学院科技创新能力建设专项(KJCX202-00103);北京市农林科学院青年基金项目(QNJJ201910);国家重点研发计划项目(2020YFD1000400)。

摘　　要：灰霉病是百合的主要病害之一,严重影响百合生产,筛选高抗种质资源是百合抗病育种的重要途径之一。本研究对不同百合种质资源叶片进行离体接种,结合扫描电镜观察,并进行活性氧、相关酶活性的测定。结果表明,卷丹百合属于高感(HS)种质,岷江百合-1、岷江百合-2、杂交种2065-1属于中抗(R)种质,宜昌百合、湖北百合、Champion Diamonds、雷林石、Craming Diamonds、红唇、杂交种2065-22属于高抗(HR)种质。接种灰霉菌后,高抗种质湖北百合叶片中过氧化氢含量变化更为剧烈,同时超氧阴离子含量会出现2次峰值,而高感种质卷丹百合则只有1次峰值。接种灰霉菌后,百合叶片中超氧化物歧化酶(SOD)活性与百合抗病性具有相关性。Grey mold is one of the main diseases of lily, which seriously affects lily production. Thus, screening of high resistant lily germplasm is an important aspect of lily disease-resistant breeding. In this study, the leaves of different lily germplasm resources were inoculated in vitro, leaf structure and conidia growth condition were observed through scanning electron microscopy, and reactive oxygen species(ROS) and the related enzyme activities were determined. The results indicated that Lilium lancifolium belonged to the high-sensitivity(HS) germplasm. Lilium regale-1, Lilium regale-2 and the hybrid 2065-1 belonged to the middle resistance(R) germplasms. Lilium leucanthum, Lilium henryi, Champion Diamonds, Lellin Stone, Craming Diamonds, Red Lips, hybrid 2065-22 belonged to high resistance(HR) germplasms. After inoculation with Botrytis cinerea, the content of HOin leaves of high resistant germplasm Lilium henryi changed dramatically, and the content of superoxide anion showed two peaks, while only one peak was found in Lilium lancifolium. After inoculation with Botrytis cinerea, the activity of superoxide dismutase(SOD) in lily leaves was correlated with the disease resistance.

关 键 词：百合 灰霉菌 抗病性 活性氧 防御酶 

分 类 号：S682.2[农业科学—观赏园艺]