针刺对脑缺血再灌注损伤大鼠大脑皮质细胞焦亡的影响  

Anti-pyroptotic Effect of Acupuncture Reducing Cerebral Ischemic Reperfusion Injury in a Rat Model

作　　者：欧阳昕 廖可欣 宋渺渺 肖移生[2] 肖爱娇[2] Ouyang Xin;Liao Kexin;Song Miaomiao;Xiao Yisheng;Xiao Aijiao(Graduate School,Jiangxi University of Chinese Medicine,Nanchang 330004,China;College of Traditional Chinese Medicine,Jiangxi University of Chinese Medicine,Nanchang 330004,China)

机构地区：[1]江西中医药大学研究生院,南昌330004 [2]江西中医药大学中医学院,南昌330004

出　　处：《世界科学技术-中医药现代化》2022年第3期946-952,共7页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：国家自然科学基金委员会地区基金项目(81660819):热敏灸调控线粒体凋亡信号通路减轻新生小鼠缺氧缺血性脑损伤的效应机制研究,负责人:陈明人、肖爱娇;江西省科学技术厅重点研发计划项目(20161BBH80053):针刺调节线粒体凋亡信号通路对新生儿缺氧缺血性脑损伤模型修复的评价及作用机制,负责人:肖爱娇;江西省教育厅科学技术研究重点项目(GJJ201208):基于NF-кB信号通路研究针刺减轻新生小鼠缺氧缺血性脑损伤的抗炎作用,负责人:肖爱娇。

摘　　要：目的 观察针刺对脑缺血再灌注损伤(CIRI)大鼠大脑皮质形态结构、NLRP3表达、caspase-1蛋白表达及活性和血清白介素-1β(IL-1β)含量的影响,以阐明针刺减轻CIRI的作用机制。方法 将100只雄性SD大鼠随机分为假手术组(Sham组)、模型组(IR组)和针刺组(ACU组)。采用线栓法栓塞大脑中动脉,并于2 h后再灌注以制备CIRI模型,针刺组给予针刺“百会”“大椎”“足三里”穴治疗,每日1次,20 min/次,共4次(即再灌注后2 h、24 h、48 h、72 h);HE染色法观察大脑皮质形态结构;Western blot技术检测大脑皮质NLRP3和caspase-1表达;比色法检测大脑皮质caspase-1活性;放射免疫分析法测定血清IL-1β含量。结果与Sham组相比,IR组大鼠脑梗死灶增大,大脑皮质细胞排列疏松,可见大量炎细胞浸润,NLRP3和caspase-1蛋白表达显著增多(P<0.05),大脑皮质caspase-1活性升高(P<0.05),血清IL-1β含量增加(P<0.05);与IR组相比,ACU组大鼠脑梗死灶减小,大脑皮质细胞排列较致密,炎细胞浸润较少,NLRP3和caspase-1(P<0.05)蛋白表达减少,大脑皮质caspase-1活性降低(P<0.05),血清IL-1β含量减少(P<0.05)。结论 针刺可能通过降低大脑皮质NLRP3和caspase-1蛋白表达、降低caspase-1活性和减少IL-1β含量,减少大鼠大脑皮质细胞焦亡,从而减轻脑缺血再灌注损伤。Objective To observe the effects of acupuncture on the expression of NLRP3 and caspase-1, the activity of caspase-1, and the content of interleukin-1β(IL-1β) in a rat model of cerebral ischemic reperfusion injury, and to explore its mechanism. Methods 100 male SD rats were randomly divided into sham group, model(IR) group and acupuncture-treated(ACU) group. The focal cerebral ischemia-reperfusion injury(CIRI) model was induced by middle cerebral artery occlusion for 2 h followed by reperfusion. The ACU group was given acupuncture at Baihui(DU20),Dazhui(DU14) and Zusanli(ST36), once a day, 20 min/time, 4 times in total(i. e., 2 h, 24 h, 48 h, and 72 h after reperfusion). The morphological structure of cerebral cortex was examined by HE staining. The expressions of NLRP3and caspase-1 were detected by western blot. The activity of caspase-1 was determined with colorimetry, and the content of IL-1β was determined using radioimmunoassay.Results Compared with the sham group, rats in the IR group showed more inflammatory cell infiltration, higher expression of NLRP3(P<0.05) and caspase-1(P<0.05), higher activity of caspase-1(P<0.05) in the cerebral cortices, and higher level of IL-1β(P<0.05) in the serum. Compared with the IR group, rats in the ACU group exhibited less inflammatory cell infiltration, lower expression of NLRP3 and caspase-1(P<0.05), lower activity of caspase-1(P<0.05) in the cerebral cortices, and lower level of IL-1β(P<0.05) in the serum.Conclusion Acupuncture attenuated cerebral ischemic reperfusion injury, which may be related to reducing pyroptosis through decreasing the expression of NLRP3 and caspase-1, the activity of caspase-1 in the cerebral cortices,and the content of IL-1β in the serum.

关 键 词：针刺 脑缺血再灌注损伤 大鼠 细胞焦亡 NLRP3 CASPASE-1 IL-1Β 

分 类 号：R245.3[医药卫生—针灸推拿学]