刺五加皂苷B对冷应激小鼠下丘脑Hsp70及应激激素mRNA和蛋白表达量的影响  

Effects of Acanthopanax Saponin B on Expression of Hsp70 and Stress Hormone mRNA and Protein in Hypothalamus of Coldstressed Mice

作　　者：孙胜悦 杨连玉[1,2,3] 杨文艳 SUN Shengyue;YANG Lianyu;YANG Wenyan(College of Animal Science and Technology,Jilin Agricultural University,Changchun 130118,China;Key Laboratory of Animal Production,Product Quality and Security,Ministry of Educa?tion,Changchun 130118,China;Jilin Provincial Key Laboratory of Animal Nutrition and Feed Science,Changchun 130118,China)

机构地区：[1]吉林农业大学动物科学技术学院,长春130118 [2]动物生产及产品质量安全教育部重点实验室,长春130118 [3]吉林省动物营养与饲料科学重点实验室,长春130118

出　　处：《吉林农业大学学报》2022年第3期365-371,共7页Journal of Jilin Agricultural University

基　　金：国家自然科学基金青年科学基金项目(31302002)。

摘　　要：为探讨刺五加皂苷B对小鼠下丘脑抗冷应激的调控作用,以小鼠下丘脑为模型,应用实时荧光定量PCR和Western-blot方法,测定相关基因Hsp70、CRH、ACTH、TRH mRNA和蛋白表达量。结果显示,冷应激条件下,4 mg/kg刺五加皂苷B对小鼠下丘脑抗冷应激相关因子和激素具有上调的作用,能够显著提高Hsp70、ACTH、TRH、CRH蛋白和基因的相对表达量。说明刺五加皂苷B对冷应激小鼠下丘脑具有保护作用,此保护作用是通过诱导激活SAM系统、HPA轴和HPT轴,从而增加下丘脑中ACTH、TRH、CRH等抗应激激素含量,并且使得应激蛋白Hsp70表达增强,提高冷应激小鼠抗冷应激能力。To investigate the regulation of Acanthopanax senticosus saponin B on hypothalamus cold stress in mice,real-time fluorescent quantitative PCR and Western-blot were used to determine the expression of Hsp70,CRH,ACTH,TRH genes and protein in the hypothalamus of mice.The results showed that 4 mg/kg saponin B could up-regulate the anti-cold stress related factors and hormones in the hypothalamus of mice under cold stress conditions,and significantly increase the relative expression of Hsp70,ACTH,TRH,CRH proteins and genes.It is concluded that Acanthopanax senticosus saponin B has protective effect on hypothalamus of mice under cold stress.The protective effect of Acanthopanax senticosus saponin B is to increase the content of ACTH,TRH and CRH in hypothalamus by inducing the activation of SAM system,HPA axis and HPT axis,and to enhance the expression of stress protein Hsp70,so as to enhance the ability of cold stress mice to resist cold stress.

关 键 词：刺五加皂苷B 冷应激 HSP70 应激激素 

分 类 号：S567[农业科学—中草药栽培]