化瘀无糖颗粒HPLC指纹图谱研究  

Study on HPLC Fingerprint Chromatography of Huayu Glycoprival Granules(化瘀无糖颗粒)

作　　者：陈金娜 万齐越 刘向红[1] CHEN Jinna;WAN Qiyue;LIU Xianghong(Qilu Hospital of Shandong University,Jinan 250012,China)

机构地区：[1]山东大学齐鲁医院,山东济南250012

出　　处：《山东中医药大学学报》2022年第5期642-646,672,共6页Journal of Shandong University of Traditional Chinese Medicine

基　　金：山东省自然科学基金项目(编号:Y2006C62);第六批全国老中医药专家学术经验传承项目(编号:194050021)。

摘　　要：目的:建立化瘀无糖颗粒高效液相色谱(HPLC)指纹图谱,为其有效质量控制提供参考。方法:Waters高效液相色谱仪,Waters 2996 DAD检测器,Phenomenex Luna C_(18)(4.6 mm×250 mm,5μm)色谱柱;乙腈(A)-0.1%磷酸溶液(B)梯度洗脱,流速1.0 mL/min,检测波长203 nm,柱温25℃。利用“中药色谱指纹图谱相似度评价系统”2004A版建立12批化瘀无糖颗粒指纹图谱,确定共有峰并进行相似度评价。结果:203 nm下12批样品指纹图谱与共有模式的相似度≥0.938。化瘀无糖颗粒指纹图谱确认了21个共有峰,指认10个共有峰,分别为次黄嘌呤(1号峰)、咖啡酸(4号峰)、香蒲新苷(6号峰)、异鼠李素-3-O-新橙皮苷(9号峰)、落新妇苷(10号峰)、人参皂苷Rg1(13号峰)、人参皂苷Re(14号峰)、人参皂苷Rb1(17号峰)、甘草酸铵(20号峰)、柴胡皂苷a(21号峰)。结论:该方法准确简便,专属性强,重复性好;所建立的指纹图谱出峰数目、出峰强度及共有峰整体分离度表现良好,适用于化瘀无糖颗粒的综合评价和质量控制。Objective:To establish the HPLC fingerprint chromatogram of Huayu Glycoprival Granules(化瘀无糖颗粒)and provide reference for its effective quality control.Methods:Waters HPLC System,Waters 2996 DAD detector,Phenomenex Luna C_(18) column(4.6 mm×250 mm,5μm),mobile phase acetonitrile-0.1% phosphoric acid solution gradient elution,flow rate 1.0 mL·min^(-1),the detection wavelength was set at 203 nm and column temperature was maintained at 25℃.The software of“TCM chromatographic fingerprint similarity evaluation system”(2004A version)was used to establish 12 batches of Huayu Glycoprival Granules fingerprints,and the common peaks were determined and the similarity was evaluated.Results:The fingerprint similarity among the samples was all over 0.938.Total 21 common peaks and 10 common peaks were identified by HPLC fingerprint,namely hypoxanthine(No.1),caffeic acid(No.4),typhaneoside(No.6),isorhamnetin-3-O-neohesperidin(No.9),astilbin(No.10),ginsenoside Rg1(No.13),ginsenoside Re(No.14),ginsenoside Rb1(No.17),ammonium glycyrrhetate(No.20)and saikoside a(No.21).Conclusions:The method is accurate,simple,specific and reproducible.The number of peaks,the intensity of peaks and the overall separation degree of common peaks established by the fingerprint shows good performance,which is suitable for the comprehensive evaluation and quality control of Huayu Glycoprival Granules.

关 键 词：化瘀无糖颗粒 高效液相色谱 指纹图谱 相似度评价 落新妇苷 质量控制 

分 类 号：R286.0[医药卫生—中药学]