细胞连续流悬浮培养技术和抗原浓缩纯化技术在口蹄疫疫苗生产中的应用  

Application of cell suspension culture and antigen purification techniques in the production of foot-and-mouth disease vaccine

作　　者：辛俊宝 胡晗[2] 赵权 张起伟 杨军义 史永贵 刘海芳 倪春霞 郭纪珂 隋心 黄建斌 李涛涛 丛日华[3] XIN Junbao;HU Han;ZHAO Quan;ZHANG Qiwei;YANG Junyi;SHI Yonggui;LIU Haifang;NI Chunxia;GUO Jike;SUI Xin;HUANG Jianbin;LI Taotao;CONG Rihua(Yangling Jinhai Biotechnology Co.,LTD.,Yangling Shaanxi 712100,China;Shaanxi Animal Disease Prevention and Control Center,Xi’an Shaanxi 710016,China;Northwest A&F University,Yangling Shaanxi 712100,China)

机构地区：[1]杨凌金海生物技术有限公司,陕西杨凌712100 [2]陕西省动物疫病预防控制中心,陕西西安710016 [3]西北农林科技大学,陕西杨凌712100

出　　处：《中兽医医药杂志》2022年第4期12-16,共5页Journal of Traditional Chinese Veterinary Medicine

基　　金：陕西省科技厅国际科技合作计划项目(2021KWZ-22)。

摘　　要：为了获得高质量的口蹄疫疫苗,对悬浮培养技术和抗原的浓缩纯化技术进行改进优化,细胞悬浮培养方式由批式培养改为连续流悬浮培养,增加离心纯化次数和超滤系统,最终可缩短48 h以上的细胞培养时间,获得高纯度和高含量的浓缩抗原,所制备的疫苗理化性状、安全性、有效性、保存期等质量指标均获得显著改善。这一技术的改进为生产高质量的口蹄疫疫苗提供了技术支持,并可实现规模化生产。The optimized cell suspension culture technology and antigen concentration and purification technology were used in the production of foot-and-mouth disease vaccine to shorten the cell culture time,improve the production efficiency and the stability of antigen.The quality of foot-and-mouth disease vaccine was improved obviously,and the character,antigen content,emulsified particle size and immune protection of the vaccine remained unchanged after storage for 30 months.The improved continuous cell flow culture technology and antigen concentration and purification technology can prepare more stable,higher purity,higher content of vaccine antigen and safe,effective,stable and long shelf life of foot-and-mouth disease vaccine products,and can realize large-scale production.

关 键 词：口蹄疫灭活疫苗 连续流细胞悬浮培养 抗原浓缩纯化 疫苗质量检测 

分 类 号：S851.3[农业科学—预防兽医学]