A label-free lateral offset spliced coreless fiber MZI biosensor based on hydrophobin HGFI for TNF-α detection  

作　　者：WANG Bo DUAN Shaoxiang ZHANG Hao XU Haijin LIU Bo QIAO Mingqiang 

机构地区：[1]The Key Laboratory of Molecular Microbiology and Technology,Ministry of Education,Nankai University,Tianjin 300071,China [2]Institute of Modern Optics,Tianjin Key Laboratory of Optoelectronic Sensor and Sensing Network Technology,Nankai University,Tianjin 300350,China [3]School of Life Science,Shanxi University,Taiyuan 030006,China1

出　　处：《Optoelectronics Letters》2022年第5期263-268,共6页光电子快报（英文版）

基　　金：supported by the Sino-Swiss Scientific and Technological Cooperation Project supported by the Ministry of Science and Technology of China(No.2015DFG32140);the National Key Research and Development Program of China(Nos.2018YFA0900100 and 2018YFB1802302);the National Natural Science Foundation of China(Nos.11774181,11804250,11904180,11904262,61875091 and 62105164);the Natural Science Foundation of Tianjin City(Nos.19JCYBJC16700,20JCQNJC01480,21JCQNJC00210 and 21JCYBJC00080);the Tianjin Education Commission Scientific Research Project(Nos.2018KJ146 and 2019KJ016).

摘　　要：A real-time label-free lateral offset spliced coreless fiber(CF)Mach-Zehnder interferometer(MZI)biosensor functionalized with hydrophobin Grifola frondosa I(HGFI)was proposed for the detection of cytokine tumour necrosis factor alpha(TNF-α).The nanolayer self-assembled on the optical fiber surfaces by HGFI rendered the immobilization of probe TNF-αantibody and recognition of antigen TNF-α.Trifluoroacetic acid was utilized to remove the HGFI layer from the glass surface,which was validated by field emission scanning electron microscopy(FESEM)and water contact angle(WCA).Results demonstrated that the processes of HGFI modification,antibody immobilization and specific antibody detection can be monitored in real time.The proposed biosensor exhibited good specificity,repeatability and low detection limit for TNF-α,extending its application in inflammation and disease monitoring.

关 键 词：FIBER interferometer OFFSET 

分 类 号：TP212[自动化与计算机技术—检测技术与自动化装置] R318[自动化与计算机技术—控制科学与工程]