臭氧亚慢性暴露对大鼠心脏lncRNA表达谱的影响  

Effects of ozone sub-chronic exposure on lncRNA expression profiles in rat heart

作　　者：赵越 田蕾 闫峻 李康 林本成 袭著革 刘晓华 ZHAO Yue;TIAN Lei;YAN Jun;LI Kang;LIN Ben-cheng;XI Zhu-ge;LIU Xiao-hua(Institute of Environmental and Operational Medicine,Academy of Military Medical Sciences,Academy of Military Sciences,Tianjin 300050,China)

机构地区：[1]军事科学院军事医学研究院环境医学与作业医学研究所,天津300050

出　　处：《中国应用生理学杂志》2022年第3期258-263,共6页Chinese Journal of Applied Physiology

基　　金：国家重点研发计划项目(2017YFC0211600,2017YFC0211603)。

摘　　要：目的:观察臭氧亚慢性暴露后大鼠心脏中lncRNA表达变化,为探索lncRNA在臭氧亚慢性暴露致心脏损伤中的作用与机制提供科学数据。方法:将18只Wistar大鼠随机分为清洁空气组和臭氧暴露组,每组9只,置于气体染毒柜中,清洁空气组吸入过滤空气,而臭氧暴露组吸入含0.5 ppm(0.980 mg/m 3)臭氧的混合气体,每天6 h,持续90 d。染毒结束后取心脏组织并提取总RNA,利用大鼠lncRNA芯片和qRT-PCR技术检测大鼠心脏中lncRNA表达量,并通过生物信息学方法分析差异表达lncRNA的潜在功能。结果:与清洁空气组相比,臭氧暴露组大鼠心脏中lncRNA表达谱发生改变,其中167个显著上调,64个显著下调;GO分析提示显著上调的lncRNA主要参与生长发育,显著下调的lncRNA主要参与调节营养物质分解代谢;KEGG分析表明显著上调的lncRNA主要参与调控PI3K-Akt信号通路,显著下调的lncRNA主要参与调控多种维生素和主要供能物质的代谢过程。结论:臭氧亚慢性暴露可致大鼠心脏lncRNA表达谱发生变化,差异表达的lncRNA可能通过影响心脏中能量和营养物质代谢在臭氧亚慢性暴露致心脏损伤中发挥作用。Objective:This article aims to observe the changes in long noncoding RNA(lncRNA)expression profiles in rat hearts after ozone sub-chronic exposure.To provide scientific data to explore the role and mechanism of differentially expressed lncRNA in damaged hearts caused by ozone sub-chronic exposure.Methods:Eighteen Wistar rats were randomly divided into filtered air and ozone exposure groups,with nine rats in each group.The rats in filtered air group were exposed to filtered air,while the rats in ozone exposure group were exposed to ozone at 0.5 ppm(0.980 mg/m 3)for 90 days at a frequency of 6 hours per day.After ozone exposure,cardiac tissues were collected and the total RNA was extracted.The expression level of lncRNA in the hearts of two groups was detected by microarray and qRT-PCR method and the potential functions of the differentially expressed lncRNA were analyzed by bioinformatics.Results:Compared with the filtered air group,lncRNA's expression profile was significantly altered in the rat hearts of ozone exposure group.A total of 167 lncRNA were up-regulated significantly and 64 lncRNA were down-regulated significantly.GO analysis indicated that the up-regulated lncRNA might involve in the process of regulating growth and development,and the down-regulated lncRNA might participate in nutrient catabolic.KEGG results showed that the up-regulated lncRNA might be involved in regulating the PI3K-Akt signaling pathway.The down-regulated lncRNA might regulate the metabolic processes of various vitamins and main energy-supplying substances.Conclusion:Ozone sub-chronic exposure can cause changes in the expression profile of lncRNA in rat hearts,which may regulate the effects of ozone sub-chronic exposure on the heart through the metabolism of energy and nutrients.

关 键 词：臭氧 lncRNA表达谱 亚慢性暴露 大鼠 心脏 

分 类 号：R1[医药卫生—公共卫生与预防医学]