快速心房起搏对B型钠尿肽受体颗粒性膜结合鸟苷酸环化酶环磷酸鸟苷通路的影响  

Effects of rapid atrial pacing on NPR-B-PGC-cGMP signaling pathway in rabbits

作　　者：韩成勇 曹淑霞 李香丹[1] 许东元[1] 玄春花 Han Chengyong;Cao Shuxia;Li Xiangdan;Xu Dongyuan;Xuan Chunhua(School of Medicine Yanbian University,Yanji133000,Jilin Province,China)

机构地区：[1]延边大学医学院,延吉133000 [2]延边大学附属医院老年医学科

出　　处：《中华老年心脑血管病杂志》2022年第8期871-874,共4页Chinese Journal of Geriatric Heart,Brain and Vessel Diseases

基　　金：国家自然科学基金(81460056);吉林省教育厅项目(JJKH20191160KJ)。

摘　　要：目的研究心房颤动发生时B型利钠肽受体(NPR-B)、颗粒性膜结合鸟苷酸环化酶(PGC)、环磷酸鸟苷(cGMP)改变及发生机制。方法40只白兔随机分为5组:对照组、快速心房起搏(RAP)1 h组、RAP 2 h组、RAP 4 h组、RAP 8 h组,每组8只,对照组只置入电极不进行心房电刺激。分别用放射免疫实验和ELISA检测各组血浆心房钠尿肽(ANP)和cGMP表达,Western blot检测心房NPR-B表达。实验结束后,取对照组和RAP 8 h组心房进行灌流实验。结果与对照组比较,RAP 1 h组、RAP 2 h组、RAP 4 h组、RAP 8 h组血浆ANP和RAP 1 h组cGMP水平升高,RAP 8 h组cGMP水平降低(P<0.05,P<0.01),RAP 1 h组、RAP 2 h组、RAP 4 h组、RAP 8 h组心房组织NPR-B蛋白表达无明显变化(0.88±0.08,0.90±0.08,0.88±0.11,0.82±0.09 vs 0.85±0.05,P>0.05)。有C型钠尿肽时,RAP 2 h组、RAP 4 h组、RAP 8 h组PGC活性明显低于对照组(P<0.05,P<0.01)。灌流实验显示,RAP 1 h组、RAP 2 h组、RAP 4 h组、RAP 8 h组心房组织对C型钠尿肽的敏感性较对照组降低。结论心房颤动发生时NPR-B对ANP敏感性降低,导致PGC活性降低,进而引起cGMP生成减少。Objective To study the changes in NPR-B-PGC-cGMP signaling pathway during atrial fibrillation and investigate the underlying mechanism in the occurrence.Methods 40 white rabbits were randomly divided into control group,RAP 1,2,4,and 8 h groups,respectively(n=8).The rabbits from the control group were implanted with electrodes.The expression and contents of ANP and cGMP were detected by radioimmunoassay and ELISA.The atrial expression of NPR-B was detected by Western blotting.The atrium from the control and RAP 8 h group were isolated for atrial perfusion test.Results In vivo,when compared with the control group,the plasma ANP level was higher in the 4 RAP groups increased(P<0.05),while the cGMP level was increased first(the RAP 1 h group)and then decreased with the elongation of stimulation,and finally(the RAP 8 h group),the level was lower than that of the control group(P<0.05,P<0.01).There were no significant differences in the expression level of NPR-B in the RAP 1 h,2 h,4 h and 8 h groups when compared with the control group(0.88±0.08,0.90±0.08,0.88±0.11,0.82±0.09 vs 0.85±0.05,P>0.05).In the presense of CNP,the activity of PGC was obviously lower in the RAP 2 h,4 h and 8 h groups than the control group(P<0.05,P<0.01).Atrial perfusion test showed that the atrial tissue exhibited lower sensitivity to CNP in the 4 RAP groups than the control group.Conclusion When atrial fibrillation occurs,NPR-B is less sensitive to ANP,which resulting in reduced PGC activity and thus reduced cGMP production.

关 键 词：心房颤动 鸟苷酸环化酶 磁性纳米粒子 RNA 长链非编码 

分 类 号：R541.75[医药卫生—心血管疾病]