TcYellow-h基因过表达参与介导赤拟谷盗对磷化氢的抗性形成  

Over-Expression of Tcyellow-H Gene Involved in Phosphine Resistance in Tribolium castaneum

作　　者：陈二虎 孟宏杰 陈艳 宋伟 唐培安 Chen Erhu;Meng Hongjie;Chen Yan;Song Wei;Tang Peian(College of Food Science and Engineering,Collaborative Innovation Center for Modern Grain Circulation and Safety,Key Laboratory of Grains and Oils Quality Control and Processing,Nanjing University of Finance and Economics,Nanjing 210023)

机构地区：[1]南京财经大学食品科学与工程学院,江苏省现代粮食流通与安全协同创新中心,江苏高校粮油质量安全控制及深加工重点实验室,南京210023

出　　处：《中国粮油学报》2022年第7期16-21,共6页Journal of the Chinese Cereals and Oils Association

基　　金：国家重点研发计划(2019YFC1605303,2016YFD0401004-04),江苏省高校自然科学研究面上项目(17KJB210005),江苏省六大人才高峰高层次人才项目(NY-057),江苏省高校优势学科建设工程资助(YXK1706)。

摘　　要：以赤拟谷盗为研究对象,旨在明确表皮相关基因TcYellow-h在害虫磷化氢抗性形成过程中的作用。序列分析结果表明TcYellow-h编码468个氨基酸,其拥有一段保守的MRJP(major royal jelly protein)特征序列;RT-qPCR结果显示,TcYellow-h在赤拟谷盗外周组织器官(头、胸、腹的表皮、翅和足)中表达水平较高,在内部器官(脂肪体、肠道、马氏管)中的表达量相对较低;此外,在不同地理种群中,TcYellow-h的表达量随磷化氢抗性水平的增加呈上升趋势;注射dsRNA能有效抑制TcYellow-h基因的表达,沉默效率为73.0%;干扰抗性种群(GD)TcYellow-h基因的表达后再用LC_(30)的磷化氢处理试虫,赤拟谷盗的死亡率显著上升至64.4%。本研究显示赤拟谷盗表皮相关基因Yellow-h的过表达与磷化氢的抗性形成相关。The present study took T.castaneum as the research object to clarify the role of cuticle related gene TcYellow-h in the formation of phosphine resistance.The sequence analysis demonstrated that TcYellow-h gene encoded proteins with 468 amino acid residues,and it had a conserved MRJP(major royal jelly protein)motif.The RT-qPCR analysis suggested that TcYellow-h genes had a higher expression in the peripheral tissues(the cuticles of head,chest and abdomen,wings and foot)of T.castaneum,and had a lower expression in the internal tissues,such as fat body,gut and malpighian tube.Additionally,it was found that TcYellow-h genes increased their expression levels with the increasing of phosphine resistance levels in different populations of T.castaneum.Afterwards,the injection of dsRNA could significantly inhibit the expression of TcYellow-h genes,with a silencing efficiency of 73.0%.When T.castaneum were treated with phosphine(LC_(30)),the mortality of insects increased to 64.4%after the TcYellow-h genes were silenced.The present study showed that the over-expression of cuticle related gene TcYellow-h might be associated with phosphine resistance in T.castaneum.

关 键 词：赤拟谷盗 磷化氢抗性 表皮相关基因 TcYellow-h RNA干扰 

分 类 号：TS210.2[轻工技术与工程—粮食、油脂及植物蛋白工程]