单细胞测序技术及其在畜牧科学研究中的应用  

Single-cell Sequencing Technology and Its Application in Animal Science Research

作　　者：康彦东 王兴东 郭韶珂 曹梦丽 郭宪[1] KANG Yan-dong;WANG Xing-dong;GUO Shao-ke;CAO Meng-li;GUO Xian(Lanzhou Institute of Husbandry and Pharmaceutical Sciences,Chinese Academy of Agricultural Sciences,Key Laboratory of Animal Genetics and Breeding on the Tibetan Plateau for Ministry of Agriculture and Rural Affairs,Key Laboratory of Yak Breeding Engineering of Gansu Province,Lanzhou 730050)

机构地区：[1]中国农业科学院兰州畜牧与兽药研究所,农业农村部青藏高原畜禽遗传育种重点实验室,甘肃省牦牛繁育工程重点实验室,兰州730050

出　　处：《中国牛业科学》2022年第4期41-45,共5页China Cattle Science

基　　金：财政部和农业农村部——现代农业(肉牛牦牛)产业技术体系建设专项(CARS-37);中国农业科学院科技创新工程项目(25-LZIHPS-01)。

摘　　要：单细胞测序(single cell sequencing)是对单个细胞进行测序后利用生物信息学等手段进行分析的技术,包括单细胞分离、扩增测序和数据分析3个方面,主要应用于肿瘤、胚胎发育、免疫学等方面。随着单细胞测序技术的迅速发展,单细胞测序技术已成为研究细胞发育中基因组、转录组和表观基因组异质性的重要工具。单细胞测序可以获取单个细胞完整的全基因组,克服了大样本量、细胞异质性等问题,为研究细胞间异质性信息和细胞群体之间关系提供了新的研究策略。本文就单细胞测序方法及其在畜牧科学研究中的应用进行综述,并探讨其应用前景。Single-cell sequencing is essentially the analysis of a single cell using bioinformatics and other post-sequencing technologies. It is mainly used in tumors, embryonic development, immunology and other aspects. With the rapid development of single-cell sequencing technology, it has become an important tool for studying the heterogeneity of genome, transcriptome and epigenome during cell development. Single-cell sequencing, which provides the complete genome of a single cell, overcomes the problems of large sample size and cell heterogeneity and provides a new technology to study information on intercellular heterogeneity and the relationship between cell populations. The paper reviewed the sequencing methods of single cell sequencing and their application in animal production.

关 键 词：单细胞测序 异质性 畜牧科学 

分 类 号：S8-1[农业科学—畜牧兽医]