雄激素受体缺乏抑制肝脏Vtg合成导致卵巢发育障碍  

ANDROGEN RECEPTOR DEFICIENCY CAUSES OVARIAN DYSGENESIS VIA REDUCING THE HEPATIC VITELLOGENIN PRODUCTION IN ZEBRAFISH

作　　者：李天慧 翟刚 贺江燕[1] 娄气永 金霞[1] 杜震宇[3] 肖武汉[1,2] 殷战 LI Tian-Hui;ZHAI Gang;HE Jiang-Yan;LOU Qi-Yong;JIN Xia;DU Zhen-Yu;XIAO Wu-Han;YIN Zhan(State Key Laboratory of Freshwater Ecology and Biotechnology,Institute of Hydrobiology,Chinese Academy of Sciences,Wuhan 430072,China;College of Advanced Agricultural Sciences,University of Chinese Academy of Sciences,Beijing 100049,China;LANEH,School of Life Sciences,East China Normal University,Shanghai 200241,China;The Innovative Academy of Seed Design,Chinese Academy of Sciences,Wuhan 430072,China)

机构地区：[1]中国科学院水生生物研究所淡水生态与生物技术国家重点实验室,武汉430072 [2]中国科学院大学,北京100049 [3]华东师范大学生命科学学院,上海200241 [4]中国科学院种子创新研究院,武汉430072

出　　处：《水生生物学报》2022年第9期1277-1284,共8页Acta Hydrobiologica Sinica

基　　金：国家重点研发计划“蓝色粮仓科技创新”重点专项(2018YFD0900205);国家自然科学基金(31972779);中国科学院计划-战略性先导科技专项A(XDA24010206);中国科学院青年创新促进会(2020336);淡水生态与生物技术国家重点实验室项目(2016FBZ05)资助。

摘　　要：为研究雄激素受体(Androgen receptor,Ar)在卵巢发育中的作用机制,文章以雄性激素受体敲除的雌性斑马鱼(Danio rerio;ar^(–/–))为研究对象,利用ELISA和蛋白质免疫印迹等方法分析Ar对斑马鱼肝脏中卵黄蛋白原(Vitellogenin,Vtg)产生、母体营养通过Vtg运输、卵黄形成和卵巢成熟的影响。研究发现在ar^(–/–)雌性斑马鱼的肝脏中,Vtg的产生和雌激素受体的表达水平显著降低。在ar^(–/–)雌性斑马鱼中的卵巢质量、脂质含量和类胡萝卜素含量均显著下降,表明Ar的缺失可导致雌性斑马鱼通过Vtg转运到卵巢的脂质和类胡萝卜素等营养物质供应减少。此外,ar^(–/–)雌性斑马鱼中与卵巢发育相关的几个基因转录表达水平显著下调,这与卵巢发育异常存在相关性。研究结果表明,Ar的缺乏可通过对肝脏中的雌激素受体表达的影响,降低肝脏Vtg蛋白的合成,从而损害由Vtg向卵巢的营养物质运输和卵黄的正常形成,影响卵巢发育。研究阐明了雄激素信号通路与体内肝脏Vtg产生之间的联系。It has been observed the ovarian dysgenesis exhibited in androgen receptor(ar)knockout female zebrafish,with significant decreased gonadosomatic index(GSI)and expression levels of lhcgr and foxl2,reflecting reduced pro-portion of mature oocytes.However,the mechanism relating to the androgen receptor in ovarian development is still unknown.The mechanism of ar-deficiency for the ovarian dysgenesis in female zebrafish has been further explored in this study.The significantly reduced levels of vitellogenin(Vtg)production and estrogen receptor expressions have been seen in the liver of ar^(–/–)females.The ovarian mass,lipid content and carotenoid content decreased significantly,indicates a reduced supply of nutrients such as lipid and carotenoids transferred via Vtg to the ovary in ar^(–/–)females.Besides,the correlation between the significantly downregulated transcriptional expression levels of several key genes related with the ovarian development and the stagnation of ovarian development has been observed in the ar^(–/–)females.Our results demonstrate that the ar-deficiency downregulates the estrogen receptors in the liver,impairs the Vtg trans-portation and yolk formation in the ovaries,and ultimately disrupts ovarian development.This study shed light on the connection between the androgen signaling and hepatic Vtg production in vivo.

关 键 词：雄激素受体 卵黄原 卵巢发育障碍 卵黄形成 斑马鱼 

分 类 号：Q344.1[生物学—遗传学]