多环芳烃(萘)与聚苯乙烯微球联合对普通小球藻的急性毒性效应  

ACUTE TOXIC EFFECTS OF POLYCYCLIC AROMATIC HYDROCARBONS(NAPHTHALENE)COMBINED WITH POLYSTYRENE MICROSPHERES ON CHLORELLA VULGARIS

作　　者：张潇峮[1] 李立杰 彭梦 柴毅[2] 张辉[1] 罗鸣钟[2] ZHANG Xiao-Qun;LI Li-Jie;PENG Meng;CHAI Yi;ZHANG Hui;LUO Ming-Zhong(Key Laboratory of Freshwater Biodiversity Conservation,Ministry of Agriculture and Rural Affairs of China,Yangtze River Fisheries Research Institute,Chinese Academy of Fishery Sciences,Wuhan 430223,China;College of Animal Science/Agriculture,Yangtze University,Jingzhou 434025,China)

机构地区：[1]中国水产科学研究院长江水产研究所,农业农村部淡水生物多样性保护重点实验室,武汉430223 [2]长江大学动物科学学院/农学院,荆州434020

出　　处：《水生生物学报》2022年第9期1364-1373,共10页Acta Hydrobiologica Sinica

基　　金：农业农村部淡水生物多样性保护重点实验室开放课题(LFBC1011);湖北省重点研发计划(2020BBB113)资助。

摘　　要：为了解多环芳烃类污染物与微塑料的联合毒性效应,实验通过检测藻密度、叶绿素a含量(Chl.a)、总蛋白(TP)、丙二醛含量(MDA)、过氧化氢酶活性(CAT)和超氧化物歧化酶活性(T-SOD)等指标,探究了环境中持久型污染物萘(Nap)和5μm聚苯乙烯微塑料(MPs)联合胁迫对普通小球藻96h的急性毒性效应。结果表明:Nap和MPs单独和联合胁迫时对普通小球藻均有抑制作用,单独胁迫时最高抑制率分别为68.26%和57.40%,联合胁迫时最高抑制率为52.05%;对Chl.a也均产生抑制作用,与藻密度呈现出较好的一致性。在单独及联合胁迫下,TP含量均显著下降,MDA含量均显著上升。Nap单独胁迫时CAT和T-SOD活性均呈显著下降趋势;MPs单独胁迫和联合胁迫时,CAT和T-SOD活性均先上升后下降,且联合胁迫实验组最终CAT和T-SOD活性均显著高于对照组。Nap和MPs对普通小球藻的EC_(50)-96h分别为81.35和383.3 mg/L,Nap较MPs对普通小球藻的毒性更强;Nap和MPs(毒性单位比1﹕1)对普通小球藻的EC_(50)-96h为1.320 TU,联合毒性效应表现为拮抗作用。可见,MPs的存在降低了Nap的毒性,在短期内体现出一定的保护作用。Naphthalene(Nap),as one of the main types of polycyclic aromatic hydrocarbons pollutants,widely exists in China’s aquatic environment.Micro-plastics(MPs)can easily become an excellent carrier of persistent organic pollu-tants and form compound toxic effects on aquatic organisms.However,there are few studies on the acute toxic effects of these two pollutants combined.In order to understand the joint toxic effects of polycyclic aromatic hydrocarbons(PAHs)and microplastics,the algae density,chlorophyll a(Chl.a),Soluble protein(TP),malondialdehyde content(MDA),catalase activity(CAT)and superoxide dismutase activity(T-SOD)were measured for studying the acute toxic effects of Nap combined with MPS on Chlorella vulgaris,which is an indicator of ecological environment.The results showed that C.vulgaris could be inhibited by both nap and MPs.The highest inhibition rates were 68.26%and 78.36%respectively under single stress,and 52.05%under combined stress.The inhibitory effect on Chl.a was consistent with the algae density.Under single and combined stress,TP content decreased and MDA content increased significantly.The activities of CAT and T-SOD decreased significantly under Nap stress.The activities of CAT and T-SOD in-creased first and then decreased under MPs and combined stress.The final activities of CAT and T-SOD of the com-bined stress experimental group were significantly higher than the control group.The EC_(50)-96h of Nap and MPs on C.vulgaris were 81.35 and 383.3 mg/L,respectively.The toxicity of Nap was stronger than MPs.The EC_(50)-96h of Nap combined with MPs(toxic unit ratio 1﹕1)on C.vulgaris was 1.320 TU.The results showed that joint toxic effect of the two pollutants hasn’t been more significant than single pollutant,and the joint toxic effect on C.vulgaris was antagoni-stic.MPs played an adsorption polymerization role on Nap,which greatly reduced the probability of contact between both pollutants and C.vulgaris.The existence of MPs showed a certain protective effect in the short term.

关 键 词：萘 微塑料 联合胁迫 急性毒性效应 普通小球藻 

分 类 号：Q178.1[生物学—水生生物学]