钌-石墨烯量子点人工酶合成及用于胡萝卜中辛硫磷的光度检测  

Synthesis of Ruthenium-Graphene Quantum Dots Artificial Oxidase and Its Application in Colorimetric Detection of Phoxim in Carrots

作　　者：王文栋 李在均 WANG Wen-Dong;LI Zai-Jun(School of Chemistry and Materials Engineering,Jiangnan University,Wuxi 214125,China)

机构地区：[1]江南大学化学与材料工程学院,无锡214125

出　　处：《应用化学》2022年第8期1285-1293,共9页Chinese Journal of Applied Chemistry

基　　金：国家重点研发计划项目(No.2018YFC1603001)资助。

摘　　要：组氨酸功能化石墨烯量子点(His-GQDs)与三氯化钌反应生成稳定的钌配合物。此配合物在氮气氛围中于600℃恒温处理1 h得到钌-石墨烯量子点复合物(Ru-His-GQD)。扫描电子显微镜(SEM)和透射电子显微镜(TEM)分析的结果表明,Ru-His-GQD具有三维结构。钌纳米粒子粒径位于40~60 nm之间。Ru-HisGQD含有丰富的功能基团,具有高的类氧化酶活性。基于Ru-His-GQD催化3,3′,5,5′-四甲基联苯胺(TMB)氧化生成蓝色化合物,建立了一种测定胡萝卜中辛硫磷的光度分析方法。辛硫磷可抑制Ru-His-GQD氧化酶的活性,导致蓝色化合物吸光度下降。当辛硫磷浓度在30~240μg/L之间,TMB氧化产物在652 nm处的吸光度随辛硫磷浓度增加而线性下降。方法的检出限达到7.33μg/L(S/N=3),灵敏度高于现有文献报道结果,成功用于胡萝卜中辛硫磷的检测。The histidine functionalized graphene quantum dots(His-GQDs)react with ruthenium trichloride to form a stable ruthenium complex.This complex is treated in a N2atmosphere at 600℃for 1 h to obtain a ruthenium-graphene quantum dot composite(Ru-His-GQD).The results of scanning electron microscopy(SEM)and transmission electron microscopy(TEM)analysis demonstrate that Ru-His-GOD has one threedimensional structure.The diameter of ruthenium nanoparticles is between 40 and 60 nm.Ru-His-GQD is rich in functional groups and has high oxidase-like activity.Based on Ru-His-GQD catalyzed oxidation of 3,3′,5,5′-tetramethylbenzidine(TMB)to produce blue compounds,a photometric method for the determination of phoxim in carrots is established.Phoxim can inhibit the activity of Ru-His-GQD oxidase,resulting in a decrease in the absorbance of the blue compound.When the concentration of phoxim is between 30~240μg/L,the absorbance of the oxidation product of TMB at 652 nm decreases linearly with the increase of phoxim concentration.The detection limit of the method reaches 7.33μg/L(S/N=3),and the sensitivity is higher than those in literature.It has been successfully applied to the detection of phoxim in carrots.

关 键 词：农药检测 功能化石墨烯量子点 钌纳米粒子 辛硫磷 比色传感器 

分 类 号：O655[理学—分析化学]