羊栖菜多糖对3T3-L1前脂肪细胞增殖的影响及其机制  

Effect and Mechanism of SFPS on 3T3-L1 Preadipocytes Proliferation

作　　者：杨旭东[1] 石杰[1] 杨骄霞 王桂云[3] 宋高臣[3] 张杰[3] YANG Xu-Dong;SHI Jie;YANG Jiao-Xia;WANG Gui-Yun;SONG Gao-Chen;ZHANG Jie(Department of Pharmacology,Mudanjiang Medical College,Mudanjiang 157011,China;Department of Cardiology,HongQi Hospital Affiliated to Mudanjiang Medical College,Mudanjiang 157011,China;Department of Biochemistry,Mudanjiang Medical College,Mudanjiang 157011,China)

机构地区：[1]牡丹江医学院药理教研室,黑龙江牡丹江157011 [2]牡丹江医学院附属红旗医院心内科,黑龙江牡丹江15701 [3]牡丹江医学院生化教研室,黑龙江牡丹江1570111

出　　处：《中国药物经济学》2022年第7期101-104,共4页China Journal of Pharmaceutical Economics

基　　金：黑龙江省省属高等学校基本科研业务费科研项目(2018-KYYWFMY-0035)。

摘　　要：目的 探讨羊栖菜多糖(SFPS)对3T3-L1前脂肪细胞增殖及凋亡的作用及其机制。方法 采用MTT法测定不同浓度SFPS对3T3-L1前脂肪细胞增殖活性的影响,应用Hoechst33258染色法检测细胞凋亡情况,实时荧光定量逆转录-聚合酶链反应(RT-PCR)检测Bax、Bcl-2和Caspase-3基因m RNA表达量的变化。结果 与对照组比较,不同浓度SFPS可降低3T3-L1前脂肪细胞增殖活力,诱导细胞凋亡,明显降低Bcl-2基因m RNA表达(P<0.05),明显增高Bax和Caspase-3基因m RNA表达(P<0.05)。结论 SFPS能够抑制3T3-L1前脂肪细胞增殖活性,其作用机制可能与诱导细胞凋亡,调控相关基因Bcl-2、Bax和Caspase-3 mRNA的表达有关。Objective To explore the effect and mechanism of SFPS on 3T3-L1 preadipocytes proliferation and apoptosis.Methods MTT assay was used to determine the effect of different concentrations of SFPs on the proliferation of 3T3-L1preadipocytes.Hoechst33258 staining was used to detect the apoptosis.Real time fluorescence quantitative reverse transcription polymerase chain reaction(RT-PCR) was used to detect the changes of Bax,Bcl-2 and caspase-3 gene mRNA expression.Results Different concentration of SFPS could inhibite proliferation of 3T3-L1 preadipocytes,and induce apoptosis of 3T3-L1preadipocytes.SFPS could effectively reduce the level of Bcl-2 gene mRNA expression and improve the level of Caspase-3 and Bax gene mRNA expression.Conclusion SFPS could inhibit proliferation of 3T3-L1 preadipocytes,and the mechanism might be that SFPS significantly induced apoptosis through regulating Bax、Bcl-2 and Caspase-3 gene expression.

关 键 词：羊栖菜多糖 3T3-L1前细胞 凋亡 基因表达 

分 类 号：R966[医药卫生—药理学]