红景天苷改善慢性阻塞性肺疾病炎症模型及气道重塑的机制  

Mechanism of salidroside improving inflammation and airway remodeling in rats with chronic obstructive pulmonary disease

作　　者：黄莺[1] 周芬 袁文胜[1] 徐芳[1] HUANG Ying;ZHOU Fen;YUAN Wensheng;XU Fang(Department of Respiratory and Criti-cal Care Medicine,Wuhan First Hospital,Wuhan 430022,China)

机构地区：[1]武汉市第一医院呼吸与危重症医学科,武汉430022 [2]泰康同济(武汉)医院呼吸与危重症医学科,武汉430022

出　　处：《实用医学杂志》2022年第14期1753-1758,共6页The Journal of Practical Medicine

基　　金：湖北省卫生和计划生育委员会科研项目(编号:WJ2015MB238)。

摘　　要：目的探讨红景天苷(SAL)调节改善大鼠慢性阻塞性肺疾病(COPD)相关炎症及气道重塑的作用及其可能机制。方法烟熏法建立COPD大鼠模型,随机分为正常组、模型组、SAL低、高剂量组和阳性对照组,观察大鼠一般状况,用小动物肺功能检测仪检测各组大鼠肺功能;ELISA法测定大鼠血清炎症因子;HE染色观察大鼠肺组织病理;Masson染色观察气道平滑肌与胶原面积;Western blot检测肺组织中过氧化物酶体增殖物激活受体-γ(PPAR-γ)/转化生长因子β1(TGF-β1)/果蝇母源抗生物皮肤生长因子(Smad)2/3通路蛋白表达。结果与正常组比较,模型组大鼠FVC、PEF和血清IL-10水平降低,血清IL-8水平升高,支气管壁、间隔和血管周围有大量炎性细胞浸润,肺泡炎症评分升高,平滑肌明显增生、肥大,气道胶原沉积明显增多,WAm/Pbm、Wcol/Pbm值升高,肺组织p-PPAR-γ蛋白表达上调,TGF-β1、p-Smad2/3蛋白表达下调(P<0.05)。经红景天苷各剂量干预后,大鼠FEV0.1/FVC、PEF值和血清IL-10水平升高,血清IL-8水平降低,支气管壁、间隔和血管周围炎性细胞浸润减少,肺泡炎症评分降低,平滑肌增生、肥大情况改善,气道胶原沉积减少,WAm/Pbm、Wcol/Pbm值降低,肺组织p-PPAR-γ蛋白表达下调、TGF-β1、p-Smad2/3蛋白表达上调(P<0.05)。结论红景天苷可减轻大鼠COPD相关炎症,逆转气道重塑,可能与调节PPAR-γ/TGF-β1/Smad2/3通路有关。Objective To investigate the effect of salidroside(SAL)regulation on improving inflammation and airway remodeling associated with chronic obstructive pulmonary disease(COPD)in rats and its possible mechanism.Methods The COPD rat model was established by smoking method and randomly divided into normal group,model group,SAL low-dose and high-dose groups,positive control group.The general condition of the rats was observed,and the pulmonary function of the rats in each group was detected by the small animal pulmonary function tester;the serum inflammatory factors of the rats were detected by ELISA.The pathology of the rat lung tissue was observed by HE staining.The area of airway smooth muscle and collagen was observed by Masson staining.Western blot detection of peroxisome proliferator-activated receptor-γ(PPAR-γ)/transforming growth factorβ1(TGF-β1)/Drosophila maternally derived skin growth factor(Smad)2/3 pathway in lung tissue Protein.Results Compared with the normal group,the levels of FEV0.1/FVC,PEF and serum IL-10 in the model group were decreased,and the serum IL-8 level was increased.There were a large number of inflammatory cells infiltration in the bronchial wall,septum and surrounding blood vessels,the alveolitis score was increased,and the smooth muscle was significantly increased.Hyperplasia and hypertrophy,significantly increased airway collagen deposition,increased WAm/Pbm,Wcol/Pbm values,up-regulated p-PPAR-γprotein expression in lung tissue,downregulated TGF-β1,p-Smad2/3 protein expression(P<0.05).After the intervention of various doses of SAL,the rats′FEV0.1/FVC,PEF value and serum IL-10 level increased,serum IL-8 level decreased,bronchial wall,septum and perivascular inflammatory cell infiltration decreased,and alveolitis score decreased,smooth muscle hyperplasia and hypertrophy improved,airway collagen deposition decreased,WAm/Pbm,Wcol/Pbm values decreased,p-PPAR-γprotein expression in lung tissue was down-regulated,TGF-β1,p-Smad2/3 protein expression was up-regulated(P<0.05).Conclusio

关 键 词：红景天苷 慢性阻塞性肺疾病 炎症 气道重塑 作用机制 

分 类 号：R285.5[医药卫生—中药学]