加减知柏地黄丸治疗女童中枢性性早熟的疗效与雌激素受体基因多态性的关系  

Relationship between therapeutic effect of modified Zhibaidihuang pills on central precocious puberty in girls and estrogen receptor gene polymorphism

作　　者：张国强[1] 张文金[1] 李金柳[1] 陈凤媚[1] 张畅斌[1] 郑淑华[1] 刘嘉芬[1] 吕栩再[1] 卢雪银 ZHANG Gouqiang;ZHANG Wenjin;LI Jinliu;CHEN Feng-mei;ZHANG Changbing;ZHENG Shuhua;LIU Jiafeng;LV Xuzai;LU Xueyin(Guangdong Province Hospital for Women and Children Healthcare,Guangzhou 511442,China)

机构地区：[1]广东省妇幼保健院,广州511442 [2]广州中医药大学,广州510006

出　　处：《实用医学杂志》2022年第13期1690-1694,共5页The Journal of Practical Medicine

基　　金：广东省中医药局科研项目(编号:20172020)。

摘　　要：目的研究加减知柏地黄丸治疗女童中枢性性早熟的疗效与雌激素受体(ER)α、β基因多态性之间的相关性。方法选取2017年7月至2020年1月广东省妇幼保健院中医科门诊治疗的中枢性性早熟女童,根据加减知柏地黄丸的疗效将患者分为有效组60例和无效组20例。应用聚合酶链反应-限制性片段长度多态性(polymerise chain reaction-restriction fragment length polymorphism,PCR-RFLP)分析技术,检测两组女孩雌激素受体α基因PvuⅡ和XbaⅠ及受体β基因RsaⅠ和AluⅠ遗传多态性。结果两组ERα-PvuⅡ和ERα-XbaⅠ基因多态性差异无统计学意义(P>0.05);两组ERβRsaⅠ与ERβAluⅠ基因多态性差异有统计学意义(P<0.05)。结论ERα-PvuⅡ与ERα-XbaⅠ的基因多态性不影响加减知柏地黄丸治疗女童中枢性性早熟的疗效,而ERβRsaⅠ与ERβAluⅠ不同的基因多态性则对疗效有影响。Objective To study the correlation between the effect of Zhibaidihuang pills on central precocious puberty in girls and the polymorphism of estrogen receptor(ER)αandβgenes.Methods A total of 60 girls with central precocious puberty were selected from the outpatient department of traditional Chinese medicine of Guangdong Province Hospital for Women and Children Healthcare from July 2017 to January 2020.The patients were divided into effective group(n=60)and ineffective group(n=20)according to the effect of modified Zhibaidihuang pills.Polymerise chain reaction-restriction fragment length polymorphism(PCR-RFLP)was used to analyze the restriction fragment length.Genetic polymorphisms of estrogen receptorαgenes PvuⅡand Xba I and receptorβgenes RsaI and AluI were detected in the two groups of girls.Results There was no significant difference in ERα-Pvu II and ERα-XbaI gene polymorphisms between the effective group and the ineffective group(P>0.05).There were significant differences in ERβRsaI and ERβAlui gene polymorphisms between the effective group and the invalid group(P<0.05).Conclusion The genetic polymorphisms of ERα-PvuII and ERα-Xba I do not affect the efficacy of the treatment of girls with precocious puberty by modified Zhibaidihuang pills,but the different gene polymorphisms of Erβ-RsaI and Erβ-AluI do.

关 键 词：中枢性性早熟 雌激素受体Α 雌激素受体Β 基因多态性 治疗效果 

分 类 号：Q344.12[生物学—遗传学] R242[医药卫生—中医临床基础]