UHPLC-Q-TOF-MS技术结合多元统计分析研究菟丝子盐炙前后化学成分变化  

Chemical profiling of raw product of Semen Cuscuta and stir-baking Semen Cuscuta with salt solution based on UHPLC-Q/TOF-MS combined multivariate statistical analysis

作　　者：黄玉 余欢迎 熊俊伟 丁西萌 徐白杨 刘利娜 储继军[2] 吴德玲 金传山 李伟东[5] 蔡宝昌[5] 汪小莉[1,3,4] HUANG Yu;YU Huan-ying;XIONG Jun-wei;DING Xi-meng;XU Bai-yang;LIU Li-na;CHU Ji-jun;WU De-ling;JIN Chuan-shan;LI Wei-dong;CAI Bao-chang;WANG Xiao-li(Pharmacy Faculty,Anhui University of Traditional Chinese Medicine,Hefei 230012,China;The First Affiliated Hospital,Anhui University of Chinese Medicine,Hefei 230031,China;Anhui Province Key Laboratory of Traditional Chinese Medicine Decoction Pieces of New Manufacturing Technology,Hefei 230012,China;Processing National Chinese Medicine Processing Technology Heritage Base,Anhui University of Traditional Chinese Medicine,Hefei 230012,China;Nanjing University of Traditional Chinese Medicine,Nanjing 210023,China)

机构地区：[1]安徽中医药大学药学院,合肥230012 [2]安徽中医药大学第一附属医院,合肥230031 [3]中药饮片制造新技术安徽省重点实验室,合肥230012 [4]安徽中医药大学国家中医药管理局中药炮制传承基地,合肥230012 [5]南京中医药大学,南京210023

出　　处：《中国新药杂志》2022年第15期1542-1552,共11页Chinese Journal of New Drugs

基　　金：国家重点研发计划项目(2018YFC1707000);安徽中医药大学青年科技英才培育项目(2021qnyc04);安徽中医药大学高层次人才计划项目(20190108);安徽省自然科学基金青年项目(2008085QH409);2015年中医药部门公共卫生专项资金项目:中药炮制技术传承基地建设。

摘　　要：目的:明确菟丝子的化学成分,进一步分析菟丝子盐炙前后的差异性成分并筛选出质量标志物。方法:采用超高效液相色谱串联四极杆飞行时间质谱联用技术(UHPLC-Q/TOF-MS)分别在正、负离子模式下采集菟丝子盐炙前后的样品数据,并结合PeakView 2.1(AB SCIEX)数据分析软件和主成分分析(PCA)以及t检验等统计分析方法,对盐炙前后菟丝子的化学成分进行分析。结果:共鉴别出39种成分,分别包括黄酮类成分20种、有机酸类成分10种、甾类化合物2种、生物碱类2种、其他类化合物5种。应用Smica 14.1软件结合t检验统计分析方法从中筛选出10种主要的差异性成分。结论:本研究系统地分析了菟丝子盐炙前后的化合物,为阐明菟丝子的物质基础以及炮制机理提供了理论依据。同时该研究也初步明确了菟丝子的质量标志物,为后续的菟丝子质量评价提供理论指导。Objective: In order to clarify the chemical constituents of Semen Cuscutae, the differential components before and after salt-processing were analyzed and the quality control markers were screened out. Methods: In this study, the chemical constituents of Semen Cuscuta before and after salt-processing were analyzed by UHPLC-Q/TOF-MS in positive and negative ion modes. Meanwhile, the results were identified and statistically analyzed using PeakView 2.1(AB SCIEX) data analysis software, principal component analysis(PCA) and t-test. Results: A total of 39 components were identified including 20 flavonoids, 10 organic acids, 2 steroids, 2 alkaloids and 5 other compounds. Ten main differential components were screened out by SMICA-p 14.1 software combined with t-test. Conclusion: The compounds of Semen Cuscutae before and after salt-processing were systematically analyzed, providing a theoretical basis for elucidating the material basis and processing mechanism of the crude drug. This study also identified the quality control markers and provided theoretical guidance for the follow-up quality evaluation of Semen Cuscutae.

关 键 词：菟丝子 盐炙 化学成分 UHPLC-Q/TOF-MS 

分 类 号：R943.1[医药卫生—药剂学] R917[医药卫生—药学]