溶质载体家族15成员4在系统性红斑狼疮患者外周血单个核细胞中的表达及其临床意义  

Expression of peptide/histidine transporter solute carrier family 15 member 4 in peripheral blood mononuclear cells and its clinical significance in patients with systemic lupus erythematosus

作　　者：马新[1] 张志博 邱佳 周丽卿 武新峰[1] 史晓飞[1] Ma Xin;Zhang Zhibo;Qiu Jia;Zhou Liqing;Wu Xinfeng;Shi Xiaofei(Department of Rheumatology and Immunology,the First Affiliated Hospital and College of Clinical Medicine,Henan University of Science and Technology,Luoyang 471003,China;Department of Nephrology and Rheumatology,Kaifeng Central Hospital,Henan 475001,China)

机构地区：[1]河南科技大学第一附属医院风湿免疫科,洛阳471003 [2]河南省开封市中心医院肾病风湿科,475001

出　　处：《中华风湿病学杂志》2022年第6期373-378,共6页Chinese Journal of Rheumatology

基　　金：河南省医学科技攻关计划省部共建重点项目(SBGJ202002098);河南省高等学校重点科研项目计划(16A320072)。

摘　　要：目的探讨肽/组氨酸转运蛋白溶质载体家族15成员4(SLC15A)在SLE患者的PBMCs中的表达及其临床意义。方法选取55例SLE患者,根据SLEDAI评分标准,分为活动期SLE组和稳定期SLE组,以13例健康志愿者作为健康对照组,采用蛋白质印迹法检测各组外周血PBMCs中SLC15A4的表达情况,并记录SLE患者的临床资料及实验室指标。3组间SLC15A4的表达量的比较采用单因素方差分析(one-way ANOVA),SLC15A4表达水平与临床指标的相关性分析采用Pearson相关分析。结果活动期SLE组、稳定期SLE组、健康对照组中SLC15A4的表达水平分别为(0.96±0.19)、(0.88±0.14)、(0.78±0.24),活动期SLE组中SLC15A4的表达水平高于健康对照组(F=4.47,P=0.015);SLE患者PBMCs中SLC15A4表达水平与抗dsDNA抗体定量(r=0.29,P=0.031)、ESR(r=0.36,P=0.007)、SLEDAI评分(r=0.32,P=0.017)呈正相关,与尿蛋白定量(24 h)(r=0.45,P=0.127)和C3(r=0.20,P=0.133)无相关性。结论SLC15A4参与了SLE的发病,其在SLE患者PBMCs中的表达情况可作为临床评估SLE患者疾病活动程度的指标。Objective To investigate the expression and clinical significance of peptide/histidine transporter solute carrier family 15 member 4(SLC15A4)in peripheral blood mononuclear cells(PBMCs)of patients with systemic lupus erythematosus(SLE).Methods Fifty-five patients with SLE were divided into active SLE group and stable SLE group according to SLE disease activity index(SLEDAI)score,and 13 healthy volunteers were used as controls.The expression of SLC15A4 in PBMCs were detected by Western blot method.Moreover,the correlation between the expression of SLC15A4 and clinical and laboratory parameters of SLE patients were analyzed.The expression of SLC15a4 in the three groups was compared based on one-way analysis of variance(ANOVA),and the correlation between SLC15A4 expression level and clinical indicators was analyzed by Pearson correlation.Results The expression levels of SLC15A4 in active SLE group,stable SLE group and healthy control group were(0.96±0.19),(0.88±0.14),(0.78±0.24),respectively.The expression level of SLC15A4 in SLE with active disease was higher than that in healthy controls(F=4.47,P=0.015).In addition,the expression of SLC15A4 in PBMCs of SLE patients was positively correlated with the quantity of anti-double stranded DNA(anti-dsDNA)antibody,erythrocyte sedimentation rate(ESR)and systemic lupus erythematosus disease activity index(SLEDAI)(r=0.29,P=0.031;r=0.36,P=0.007;r=0.32,P=0.017,respectively).However,the expression of SLC15A4 in PBMCs had no significant correlation with 24-h urinary protein(r=0.45,P=0.127)and C3(r=0.20,P=0.133).Conclusion SLC15A4 is involved in the pathogenesis of SLE and its expression in PBMCs of SLE patients can be used as an index to evaluate disease activity.

关 键 词：红斑狼疮 系统性 单核细胞 疾病活动指数 溶质载体家族15成员4 

分 类 号：R593.241[医药卫生—内科学]