江西省青钱柳自然居群的群体遗传结构与基因流研究  

Population Genetic Structure and Gene Flow in Natural Populations of Cyclocarya paliurus in Jiangxi Province

作　　者：刘亚林 叶林江 寇一翾 范邓妹 张志勇[1] 程珊梅 LIU Yalin;YE Linjiang;KOU Yixuan;FAN Dengmei;ZHANG Zhiyong;CHENG Shanmei(College of Agronomy,Jiangxi Agricultural University,Nanchang 330045,China;College of Biology and Environmental Engineering,Jingdezhen University,Jingdezhen,Jiangxi 333400,China)

机构地区：[1]江西农业大学农学院,江西南昌330045 [2]景德镇学院生物与环境工程学院,江西景德镇333400

出　　处：《江西农业大学学报》2022年第4期938-947,共10页Acta Agriculturae Universitatis Jiangxiensis

基　　金：国家自然科学基金项目(31860167)。

摘　　要：【目的】青钱柳[Cyclocaryapaliurus(Batal.)Iljinsk.]是中国特有的单种属植物,具有与地理环境密切相关的遗传多样性和遗传分化。目前探究青钱柳群体遗传的研究很少,尤其是具有广泛分布的江西省这个独立生态单元。通过居群遗传学分析,能够获得其遗传多样性和遗传结构的相关信息,探究江西省青钱柳居群的遗传分化和基因流,进而选出广适性青钱柳种源,为该物种的保护开发提供科学参考。【方法】利用筛选出的8个多态性高的细胞核微卫星标记对江西省的26个自然居群283个个体进行荧光标记PCR,检测遗传多样性,推测其遗传结构,并计算不同组分之间的基因流迁移率。【结果】在江西省青钱柳自然居群中检测到较高水平的多态性(H_(E)=0.597、I=1.178),中度的遗传分化(F_(ST)=0.097)。Structure、UPGMA系统发育树和主成分分析(PCoA)聚类分析结果表明其遗传组分为3组,分别为N1、N2和N3。基于贝叶斯模拟和MCMC结果,发现这三者之间不管是历史基因流还是现代基因流都较大,且存在双向不对称的现象。【结论】江西省青钱柳自然居群遗传组分为3组,彼此之间具有较高的历史和现代基因流,而这可能是导致江西省青钱柳高水平遗传多样性的原因。另外,考虑到江西省青钱柳自然居群的遗传背景和开发利用现状,建议种质资源开发时应优先考虑遗传多样性水平高的居群。[Objective]Cyclocarya paliurus(Batal.)Iljinsk.is a monotypic species endemic to China,of which genetic diversity and differentiation are closely related to geographical environment.Few studies have fo⁃cused on the population genetic study of C.paliurus,especially in one independent ecological unit(e.g.Jiangxi Province),despite its wide distribution.Based on population genetic analysis,the information of genetic diversi⁃ty and genetic structure,and the genetic differentiation and gene flow of C.paliurus populations of Jiangxi Prov⁃ince can be obtained.The results of this study could help provenances selection and provide some suggestions for the conservation and development of this species.[Method]Eight highly polymorphic nuclear microsatellite markers were selected to fluorescence-labeled PCR amplification for 283 individuals from 26 natural popula⁃tions in Jiangxi Province.The genetic diversity and genetic structure were estimated,and the migration rates among different components was calculated based on different methods.[Result]A high level of genetic diversi⁃ty(H_(E)=0.597,I=1.178)and moderate genetic differentiation(F_(ST)=0.097)were detected in the natural populations of C.paliurus in Jiangxi Province.The results of structure,UPGMA phylogenetic tree and principal component analysis(PCoA)cluster analysis all showed that the number of genetic groups was three,namely N1,N2 and N3,respectively.Based on the results of Bayesian and MCMC simulations,we found that the historical gene flow as well as contemporary gene flow among the three groups were large with asymmetric bidirectional phenome⁃non.[Conclusion]The genetic group of the natural populations of C.paliurus in Jiangxi Province was divided into three groups,and there were high gene flow among them,which may be resulted from high historical gene flow and,together with contemporary gene flow.In view of the genetic background,and development and use sta⁃tus of the natural populations of C.paliurus in Jiangxi Province,we suggested that populations

关 键 词：青钱柳 江西省 SSR 遗传多样性 遗传结构 基因流 

分 类 号：Q948.13[生物学—植物学] S792.159[农业科学—林木遗传育种]