时差成像干式培养与传统湿式培养应用于不同年龄胚胎发育的效果  

Effects of time-lapse dry culture and conventional wet culture on embryonic development in different age females

作　　者：张豆豆 王静[1] 刘淼 卢大儒[2] 刘素英[1] ZHANG Dou-dou;WANG Jing;LIU Miao;LU Da-ru;LIU Su-ying(Reproductive Medicine Center,Zhongshan Hospital,Fudan University,Shanghai 200032,China;School of Life Sciences,Fudan University,Shanghai 200000,China)

机构地区：[1]复旦大学附属中山医院生殖医学中心,上海200032 [2]复旦大学生命科学学院,上海200000

出　　处：《中国临床医学》2022年第4期680-684,共5页Chinese Journal of Clinical Medicine

基　　金：国家自然科学基金(81971345).

摘　　要：目的评价时差成像技术(time-lapse,TL)干式培养与传统湿式培养对不同年龄胚胎发育的影响。方法回顾性分析2020年7月至2021年12月2390个周期体外胚胎培养情况。TL组共培养640个周期,传统湿式培养组(对照组)共培养1750个周期。两组女方年龄、不孕类型、不孕因素、促排卵药物(gonadotropin,Gn)用量、受精方式差异均无统计学意义。比较不同年龄女方中,两组间正常受精率、正常卵裂率、有效胚胎率、优质胚胎率、囊胚形成率和优质囊胚率。结果20~29岁:TL组有效胚胎率(75.39%vs 70.15%)、优质胚胎率(49.79%vs 41.98%)均高于对照组(P<0.01);30~34岁:TL组优质胚胎率(46.70%vs 41.43%)、囊胚形成率(57.14%vs 52.74%)、优质囊胚率(35.82%vs 32.26%)均高于对照组(P<0.05);≥40岁:TL组优质胚胎率(44.05%vs 48.96%)、囊胚形成率(34.9%vs 46.24%)及优质囊胚率(16.11%vs 21.77%)均低于对照组(P<0.05)。结论与常规湿式培养相比,女方20~34岁时,TL培养可显著提高胚胎发育成功率,女方≥40岁时则效果较差。Objective To evaluate the effects of time-lapse(TL)dry culture and traditional wet culture on embryonic development in different age female groups.Methods From July 2020 to December 2021,retrospective analysis of data from 2390 cycles of embryo culture in vitro,including 640 cycles in the TL group and 1750 cycles in the traditional wet culture group(control group).There were no significant differences in age of females,infertility type,infertility factors,dosage of gonadotropin(Gn),and fertilization methods between the two groups.The rates of fertilization,cleavage,tranfterable embryo,high-quality embryo,blastocyst,high-quality blastocyst were compared between the two groups in different age females.Results In the 20-29 year old females:the effective embryo rate(75.39%vs 70.15%)and the high-quality embryo rate(49.79%vs 41.98%)in the TL group were significantly higher than those in the control group(P<0.01).In the 30-34 year females:the high-quality embryo rate(46.70%vs 41.43%),the blastocyst formation rate(57.14%vs 52.74%),the high-quality blastocyst rate(35.82%vs 32.26%)in the TL group were significantly higher than those in the control group(P<0.05).In the≥40-year-old females:the high-quality embryo rate(44.05%vs 48.96%),the blastocyst formation rate(34.9%vs 46.24%)and the high-quality blastocyst rate(16.11%vs 21.77%)in the TL group were significantly lower than those in the control group(P<0.05).Conclusions Compared with conventional wet culture,TL culture can significantly increase the success rate of embryonic development in 20-34 years old females,while the effect is not good in the older females(≥40 years old).

关 键 词：时差成像技术 胚胎培养 囊胚培养 渗透压 

分 类 号：R711.6[医药卫生—妇产科学]