大白菜GH3基因家族全基因组鉴定及表达分析  

Genome-wide identification and expression analysis of the GH3 gene family in chinese cabbage

作　　者：李敬蕊[1] 解紫薇 卢银[1] 李娜 刘紫俊 赵建军[1] LI Jingrui;XIE Ziwei;LU Yin;LI Na;LIU Zijun;ZHAO Jianjun(College of Horticulture,Hebei Agricultural University,Key Laboratory of Innovation and Utilization of Vegetable Germplasm in Hebei Province,Collaborative Innovation Center of Vegetable Industry in Hebei Province,State Key Laboratory of North China Crop Improvement and Regulation,Baoding 071000,China)

机构地区：[1]河北农业大学园艺学院/省部共建华北作物改良与调控国家重点实验室/河北省蔬菜种质创新与利用重点实验室/河北省蔬菜产业协同创新中心,河北保定071000

出　　处：《河北农业大学学报》2022年第4期15-24,共10页Journal of Hebei Agricultural University

基　　金：国家自然科学基金(32172589);河北省人力资源和社会保障厅项目(C20200329);河北省科学技术厅项目(216Z2904G);河北省教育厅项目(BJ2019020)。

摘　　要：生长素酰胺合成酶(GH3)基因家族是生长素早期应答基因家族之一,在植物生长素信号转导过程中发挥重要作用。基于大白菜全基因组数据对大白菜GH3家族进行全基因组鉴定及生物信息学分析,结果表明,在大白菜基因组中共鉴定到45个包含GH3结构域的BrGH3家族成员;BrGH3家族成员编码氨基酸数在286~826之间,分子量介于32.06~90.77 KD之间;系统进化分析将BrGH3家族分为4类;基因结构分析发现该家族成员外显子数为2~10个;蛋白保守基序(motif)分析表明,除了BrGH3.2和BrGH3.18外,BrGH3家族成员都含有10个Motif;41个BrGH3成员在10条染色体上均有分布,4个基因未定位到染色体上;共线性分析发现29个基因参与片段复制事件,存在5个串联重复;启动子顺式作用元件分析表明,BrGH3成员含有大量光、激素、逆境和生长发育等响应元件。基于大白菜结球野生型和不结球平塌突变体结球前期叶片不同部位转录组数据,分析BrGH3差异表达,结果表明,其中BrGH3.28和BrGH3.41基因在大白菜突变体叶片不同部位的FPKM值均高于野生型,为野生型的1.22~4.83倍,且BrGH3基因家族在LF亚基因组中优势表达,初步推测BrGH3基因家族成员参与调控大白菜叶球发育。本研究为进一步研究BrGH3基因家族的功能和大白菜叶球发育机理提供参考。The GRETCHEN HAGEN 3(GH3)gene family was one of the early auxin response gene families,which plays important roles in plant auxin signal transduction.Based on the genomic data of Chinese cabbage,genome-wide identification and bioinformatics analysis of GH3 family in Chinese cabbage was carried out.The results showed that 45 BrGH3 family members containing GH3 domain were identified in Chinese cabbage genome.These genes encode proteins with 286-826 amino acid and the molecular weight ranged from 32.06 to 90.77 KD.The BrGH3 gene family was divided into four group in evolution.The gene family consists of 2-10 exons.Motif analysis showed that all members of BrGH3 family contain 10 motifs except for BrGH3.2 and BrGH3.18.BrGH3 members were distributed on 10 chromosomes,among which 4 genes were not located on chromosomes.Collinearity analysis showed that 29 genes were involved in fragment replication events and 5 tandem repeats were identified.Cis-acting elements prediction showed that the promoter of BrGH3 family contained a large number of response elements such as light,hormone,stress,growth and development.The expression of BrGH3 genes were analyzed using transcriptome data of different parts of wild-type and flat-growth mutant leaves at early heading stage of Chinese cabbage.The results showed that the FPKM value of expression of BrGH3.28 and BrGH3.41 in different parts of the mutant were 1.22-4.83 times higher than in those of the wild type,and the BrGH3 gene family was preferentially expressed in LF subgenome.It was speculated that these genes were involved in the regulation of leaf heading development in Chinese cabbage.This study provides a reference for further study on the function of the BrGH3 gene family and the mechanism of leaf heading development in Chinese cabbage.

关 键 词：大白菜 GH3基因家族 生物信息学分析 表达分析 

分 类 号：S634.1[农业科学—蔬菜学]