辣椒细胞质雄性不育突变体CMS102的败育机理及其育性遗传  

Abortion mechanism and genetic analysis of fertility traits in cytoplasmic male sterile mutant CMS102 of Capsicum annuum

作　　者：杨慧萍 潘路招 汤冰倩 邹学校[2,3,4] 戴雄泽 刘峰 YANG Huiping;PAN Luzhao;TANG Bingqian;ZOU Xuexiao;DAI Xiongze;LIU Feng(Longping Branch,Graduate School of Hunan University,Changsha Hunan 410125,China;College of Horticulture,Hunan Agricultural University,Changsha,Hunan 410128,China;ERC for Germplasm Innovation and New Variety Breeding of Horticultural Crops,Changsha,Hunan 410128,China;Key Laboratory for Vegetable Biology of Hunan Province,Changsha Hunan 410128,China;College of Horticulture,Nanjing Agricultural University,Nanjing,Jiangsu 210095,China)

机构地区：[1]湖南大学研究生院隆平分院,湖南长沙410125 [2]湖南农业大学园艺学院,湖南长沙410128 [3]园艺作物种质创新与新品种选育教育部工程研究中心,湖南长沙410128 [4]蔬菜生物学湖南省重点实验室,湖南长沙410128 [5]南京农业大学园艺学院,江苏南京210095

出　　处：《湖南农业大学学报（自然科学版）》2022年第4期423-429,共7页Journal of Hunan Agricultural University(Natural Sciences)

基　　金：国家特色蔬菜产业技术体系(CARS-24-A-15)。

摘　　要：以辣椒细胞质雄性不育突变体CMS102及其相应的恢复系ST–8为材料,对其造孢期、小孢子母细胞期、小孢子减数分裂期、四分体时期、单核小孢子期、双核小孢子期和花粉粒成熟期的花蕾进行细胞学形态观察,测定小孢子减数分裂期、四分体时期、单核小孢子期和双核小孢子期发育时期的花蕾中与育性相关的生理活性物质的含量。结果表明:CMS102败育发生在四分体时期,绒毡层过度膨大挤压四分体,使其不能正常发育成单核小孢子,属于孢子体不育;CMS102花蕾中可溶性蛋白和游离脯氨酸含量低于ST–8的,可溶性糖、MDA含量及POD酶活性高于ST–8的;构建F_(2)遗传分离群体并调查其育性,对CMS102的育性进行遗传学分析,CMS102育性恢复由1对显性基因调控。The cytoplasmic male sterile line CMS102 and the restorer line ST-8 of capsicum were used as materials.The anther cytological morphology of flower buds in sporogenic stage,microspore mother cell stage,microspore meiosis stage,tetrad stage,uninucleate microspore stage,binucleate microspore stage and pollen maturity stage were observed.The contents of physiologically active substances related to fertility in flower buds in microspore meiosis stage,tetrad stage,mononucleate microspore stage and binucleate microspore stage were determined.The results showed that CMS102 abortion occurred in the tetrad stage,and the tapetum overexpanded and squeezed the tetrad,so that it could not normally develop into monokaryon microspore,which belonged to sporophyte sterility;the contents of soluble protein and free proline in CMS102 buds were lower than those in the restorer lines,while the contents of soluble sugar,MDA content and POD enzyme activity in CMS102 materials were higher than those in restorer lines.The genetic segregation population of F_(2) was constructed and its fertility was investigated,and the fertility of CMS102 was analyzed.It was found that the fertility restoration of CMS102 materials was regulated by a pair of dominant genes.

关 键 词：辣椒 细胞质雄性不育突变体 花药细胞形态学 生理生化特征 育性 

分 类 号：S641.3[农业科学—蔬菜学]