日粮添加白酒糟酿酒酵母培养物对断奶仔猪生长和肠道结构及功能的影响  

Effects of adding Saccharomyces cerevisiae culture from distiller’s grains in diet on growth,intestinal structure and function of weaned piglets

作　　者：张大城 洪玲玲 刘碧凡 苏云 李斌 曹立红 冯泽猛[1] 印遇龙[1] ZHANG Dacheng;HONG Lingling;LIU Bifan;SU Yun;LI Bin;CAO Lihong;FENG Zemeng;YIN Yulong(Institute of Subtropical Agricultural Ecology,Chinese Academy of Sciences,Changsha,Hunan 410125,China;College of Animal Science and Technology,Hunan Agricultural University,Changsha,Hunan 410128,China;College of Life Sciences,Hunan Normal University,Changsha,Hunan 410081;Anhui Dongfang Xinxin Biotechnology Co.Ltd,Bozhou,Anhui 236800,China;College of Advanced Agriculture Sciences,University of Chinese Academy of Sciences,Beijing 100049,China)

机构地区：[1]中国科学院亚热带农业生态研究所,湖南长沙410125 [2]湖南农业大学动物科技学院,湖南长沙410128 [3]湖南师范大学生命科学学院,湖南长沙410081 [4]安徽东方新新生物技术有限公司,安徽亳州236800 [5]中国科学院大学现代农业科学学院,北京100049

出　　处：《湖南农业大学学报（自然科学版）》2022年第4期474-482,共9页Journal of Hunan Agricultural University(Natural Sciences)

基　　金：广西重点研发计划项目(2019AB19003);国家“十三五”重点研发计划项目(2016YFD0700200);安徽东方新新生物技术有限公司课题(Y640032100)。

摘　　要：选取12头体质量相近的健康断奶仔猪随机分为2组,对照组(CON组)和试验组(SDGS组)分别饲喂依据NRC(2012)仔猪营养需要标准配制的等氮等能基础日粮与4%白酒糟酿酒酵母培养物添加日粮,每组6个重复,预饲7 d后试验28 d,探索日粮添加白酒糟酿酒酵母培养物对断奶仔猪生长性能、器官指数、血清生化指标及肠道形态、肠黏膜紧密连接蛋白(TJ)和细胞因子mRNA相对表达量的影响,综合评价日粮添加白酒糟酿酒酵母培养物在断奶仔猪上的饲喂效果。结果表明:SDGS组的平均日增质量显著(P<0.05)高于CON组的,平均日采食量和料重比则无显著(P>0.05)变化;SDGS组的肝脏指数显著(P<0.05)低于CON组的,脾脏、心脏、胰脏、肾脏的器官指数则无显著(P>0.05)变化;血清生化指标(总蛋白、尿素、葡萄糖、免疫球蛋白M、免疫球蛋白G)在两组间的差异均无统计学意义(P>0.05);与CON组相比,SDGS组的肠道形态更完整、排列整齐,但空肠和回肠隐窝深度显著(P<0.05)增加,空肠绒毛高度显著(P<0.05)降低,空肠和回肠绒毛高度与隐窝深度比值均显著(P<0.01)降低,十二指肠隐窝深度有增加趋势(P=0.081),十二指肠绒毛高度、绒毛高度与隐窝深度比值则无显著(P>0.05)变化;SDGS组空肠黏膜TJ蛋白中闭合蛋白–3(Claudin–3)的mRNA相对表达量有降低趋势(P=0.091),空肠黏膜TJ蛋白中闭合小环蛋白–1(ZO–1)、闭锁蛋白(occludin)与十二指肠和回肠黏膜TJ蛋白Claudin–3、occludin、ZO–1的mRNA相对表达量则均无显著(P>0.05)变化;SDGS组十二指肠和回肠黏膜细胞因子白细胞介素–6(IL–6)的mRNA相对表达量显著(P<0.05)降低,回肠黏膜细胞因子白细胞介素–10(IL–10)的mRNA相对表达量有降低趋势(P=0.068),十二指肠黏膜细胞因子白细胞介素–1β(IL–1β)、IL–10,空肠黏膜细胞因子IL–6、IL–1β、IL–10和回肠黏膜细胞因子IL–1β的mRNA相对表达量则无显著(P>0.05)变�Twelve healthy weaned piglets with similar body weight were randomly divided into two groups,the control group(CON group)and the experimental group(SDGS group),and fed with an isonitrogenous isoenergetic basal diet formulated according to the NRC(2012)nutritional requirements standard for piglets and the 4%distiller’s grains Saccharomyces cerevisiae culture-supplemented diet,respectively,with six replicates in each group,the last time of the experiment was conducted for 28 days after 7 days of pre-feeding.The effects of dietary supplementation of Saccharomyces cerevisiae culture containing distiller’s grains on growth performance,organ index,serum biochemical indexes,intestinal morphology,and relative mRNA expression levels of intestinal mucosa tight junction(TJ)proteins and cytokines of weaned piglets were collected to comprehensively evaluate the feeding effects of dietary supplementation of Saccharomyces cerevisiae culture containing distiller’s grains on weaned piglets.The results showed that the average daily gain weight of the SDGS group was significant(P<0.05)higher than that of the CON group,and the average daily feed intake and feed/weight ratio had no significant(P>0.05)changes.The liver index of the SDGS group was significantly(P<0.05)lower than that of the CON group,while the organ indexes of spleen,heart,pancreas and kidney showed no significant(P>0.05)changes.The differences of serum biochemical indexes(total protein,urea,glucose,immunoglobulin M and immunoglobulin G)between the two groups were not statistically significant(P>0.05).Compared with CON group,the intestinal morphology of the SDGS group was more complete and aligned,but the jejunal and ileal crypt depth were significantly(P<0.05)increased,and the jejunal villus height was significantly(P<0.05)decreased.The ratios of jejunal and ileal villus height to crypt depth were all significantly(P<0.05)decreased,while the duodenal crypt depth showed an increasing trend(P=0.081),but the duodenal villus height,crypt depth and the ratio of villu

关 键 词：断奶仔猪 白酒糟酿酒酵母培养物 生长性能 血清生化指标 器官指数 肠道形态 紧密连接蛋白 细胞因子 

分 类 号：S828.5[农业科学—畜牧学]