沟叶结缕草ZmCOR410基因抗逆功能鉴定  

Function Identification of ZmCOR410 from Zoysia matrella in Resistance to Abiotic Stresses

作　　者：张晓寒 陈彦良 马欣 张珊珊 韦善君[1] ZHANG Xiaohan;CHEN Yanliang;MA Xin;ZHANG Shanshan;WEI Shanjun(College of Life and Environmental Sciences,Minzu University of China,Beijing 100081,China)

机构地区：[1]中央民族大学生命与环境科学学院,北京100081

出　　处：《华北农学报》2022年第4期53-61,共9页Acta Agriculturae Boreali-Sinica

基　　金：国家自然科学基金项目(31100570);中央民族大学大学生创新训练项目(URTP2016110017)。

摘　　要：为研究结缕草抗逆分子机制,报道了沟叶结缕草一个低温响应基因ZmCOR410的抗逆功能。基于转录组数据克隆获得了ZmCOR410基因,采用实时定量PCR技术检测了其表达的低温响应模式,将其转化拟南芥中和酵母中鉴定了其抗逆功能。结果表明,ZmCOR410基因编码区序列长927 bp,编码308个氨基酸,为酸性脱水素。在同源基因的系统进化树中ZmCOR410蛋白与耐旱性强的无芒隐子草和弯叶画眉草的同源基因亲缘关系最近。在基因组中,ZmCOR410基因编码区上游1700 bp范围内有4个DRE顺式元件核心序列,其mRNA在低温胁迫下显著积累。转化了ZmCOR410基因的拟南芥,叶片抗冻能力增强,植株在干旱和高温胁迫下的存活率提高。转化了ZmCOR410基因的酵母细胞抗高温能力增强。结果说明,ZmCOR410基因的表达产物在冷冻、高温和干旱胁迫下对细胞起保护作用,将有利于沟叶结缕草度过不良环境。In order to explore the molecular mechanism of Zoysia grass in resistance to abiotic stresses,in the present study we reported the function of a cold responding gene from Zoysia matrella designated as ZmCOR410.The sequence information of the gene was obtained on the base of transcriptome and genomic data.The expression profile of ZmCOR410 in response to low temperature was detected by qRT-PCR,and the functions of the gene under abiotic stresses were estimated in Arabidopsis and yeast by genetic transformation.The results showed that the CDS of the gene was 927 bp in length,encoding an acid dehydrin that contains 308 amino acids.In the polygenetic tree of COR410 homologues from gramineous grasses,ZmCOR410 had a close relationship with the homologues of Cleistogenes songorica and Eragrostis curvula,two species that were high tolerant to drought stress.In the genomic DNA,there were four copies of core sequence of DRE-cis element in the 1700 bp region upstream of the CDS of ZmCOR410,and its mRNA was accumulated in leaves exposed to cold.Compared to WT plants,Arabidopsis plants over expressing ZmCOR410 showed reduced injury in leaves after a freezing-temperature exposure,and showed higher survival rates under drought and high temperature stresses.Yeast cells harboring ZmCOR410 were also more tolerant to high temperature stress than the control cells.The results indicated that the product of ZmCOR410 could enhance cells resistance to freezing cold,high temperature and drought stresses,which would help Zoysia matrella going through adverse environments.

关 键 词：沟叶结缕草 COR基因 非生物胁迫 脱水素 

分 类 号：S543.03[农业科学—作物学]