喜树幼叶发育与喜树碱类化合物生物合成的相关性  

Correlation between the development of young leaves of Camptotheca acuminata and the biosynthesis of camptothecin compounds

作　　者：董淋淋 杨秉润 王燕燕[1] 张悦 于放[1] DONG Linlin;YANG Bingrun;WANG Yanyan;ZHANG Yue;YU Fang(School of Biological Engineering,Dalian Polytechnic University,Dalian 116034,China)

机构地区：[1]大连工业大学生物工程学院,辽宁大连116034

出　　处：《大连工业大学学报》2022年第4期242-245,共4页Journal of Dalian Polytechnic University

基　　金：国家自然科学基金项目(31970324).

摘　　要：喜树(Camptotheca acuminata)能够合成具有抗肿瘤作用的喜树碱(CPT)与10-羟喜树碱(10-HCPT),其积累具有组织特异性,但对于同一组织不同发育阶段的积累特性尚不清楚。利用高效液相色谱和实时荧光定量PCR分析喜树幼叶不同部位(叶片、叶基、叶柄)CPT和10-HCPT积累量以及关键酶基因相对表达量。结果发现,幼叶中CPT的积累量在叶片中最多,其次是叶基、叶柄;10-HCPT的积累量在叶柄中最多,其次是叶基、叶片;合成途径关键酶基因CaG8O、CaCYC1、Ca7-DLS、Ca7-DLGT、CaTDC 1以及CaSTR的表达均具有相似的趋势,且与CPT的积累量呈负相关,但与10-HCPT的积累量呈正相关。Camptothecin(CPT)and 10-hydroxycamptothecin(10-HCPT)with anti-tumor activity can be biosynthesized in Camptotheca acuminata,and their accumulations are tissue specific.However,the accumulation characteristics of the same tissue at different developmental stages are still unclear.The accumulations of CPT and 10-HCPT and relative expression of key enzyme genes in different parts of young leaves(leaf,leaf base,petioles)were analyzed by HPLC and qRT-PCR.The results show that the highest accumulation of CPT in young leaves is leaf,followed by leaf base and petiole.The highest accumulation of 10-HCPT is in petioles,followed by leaf base and the least in leaf.The expression pattern of the key enzyme genes(CaG8O,CaCYC1,Ca7-DLS,Ca7-DLGT,CaTDC 1 and CaSTR)also show similar tendency,which is negatively correlated with the accumulation of CPT,positively correlated with the accumulation of 10-HCPT in these three parts of young leaves.

关 键 词：喜树碱 10-羟喜树碱 酶基因 幼叶 

分 类 号：Q946.8[生物学—植物学]