转分化肾小管上皮细胞对DJ-1蛋白分泌及甲状旁腺细胞增殖的影响  

Transdifferentiated Renal Tubular Epithelial Cells Secrete DJ-1 Protein to Promote the Proliferation of Parathyroid cells

作　　者：位红兰 董骏武 WEI Hong-lan;DONG Jun-wu(Department of Nephrology,Wuhan Fourth Hospital,Wuhan Hubei 430030,China)

机构地区：[1]武汉市第四医院肾病内科,湖北武汉430030

出　　处：《湖北科技学院学报（医学版）》2022年第4期296-300,共5页Journal of Hubei University of Science and Technology(Medical Sciences)

基　　金：武汉市卫生健康委员会科研项目(WX18Q31)。

摘　　要：目的研究转分化肾小管上皮细胞是否分泌细胞外DJ-1蛋白,刺激甲状旁腺细胞增生、PTH分泌。方法TGF-β1诱导HK-2细胞转分化,ELISA检测培养液中分泌型DJ-1蛋白水平;用含分泌型DJ-1蛋白的上清或野生型分泌蛋白DJ-1培养甲状旁腺细胞,免疫共沉淀检测DJ-1与P-FGFR1的相互作用,Western blot检测P-ERK1/2的活化,CCK8法检测甲状旁腺细胞的增殖,电化学发光法检测PTH的分泌。结果TGF-β1培养72h,肾小管上皮细胞表达α-SMA升高,培养液中DJ-1蛋白表达升高;分泌型DJ-1蛋白与P-FGFR1相互作用,ERK1/2磷酸化;甲状旁腺细胞在含分泌型DJ-1蛋白上清及野生型分泌蛋白DJ-1培养下细胞增殖率显著增加、PTH分泌显著增加,且可被FGFR1抑制剂抑制。结论分泌型DJ-1蛋白可促进甲状旁腺细胞增殖。Objective To study whether the transdifferentiated renal tubular epithelial cells secrete extracellular DJ-1 protein,which stimulates the proliferation of parathyroid cells and the secretion of PTH.Methods TGF-β1 induced the transdifferentiation of HK-2 cells,and the level of secreted DJ-1 protein in the culture medium was detected by ELISA.The interaction between DJ-1 and P-FGFR1 was detected by precipitation,the activation of P-ERK1/2 was detected by Western blot,the proliferation of parathyroid cells was detected by CCK8,and the secretion of PTH was detected by electrochemiluminescence.Results After the stimulation of TGF-β1 for 72 h,renal tubular epithelial cells expressedα-SMA and secreted DJ-1 protein.DJ-1 protein interacted with P-FGFR1,and ERK1/2 was phosphorylated.Parathyroid cells cultured in supernatants containing secreted DJ-1 protein and wild-type secreted DJ-1 protein significantly increased cell proliferation rate and PTH secretion,which can be inhibited by FGFR1 inhibitors.Conclusion Secreted DJ-1 protein can promote the proliferation of parathyroid cells.

关 键 词：DJ-1蛋白 FGFR1 P-ERK1/2 甲状旁腺细胞 

分 类 号：R692.6[医药卫生—泌尿科学]